Target analyte detection using asymmetrical self-assembled monolayers

• US 6,753,143 B2
• Filed: 05/01/2001
• Issued: 06/22/2004
• Est. Priority Date: 05/01/2000
• Status: Expired due to Term

• Current Assignee: Roche Molecular Systems Inc (Roche Holding AG)
• Original Assignee: Clinical Micro Sensors Inc. (Roche Holding AG)
• Inventors: Yu, Changjun, Tao, Chunlin
• Primary Examiner(s): Siew, Jeffrey
• Application Number:
  US09/847,113
• Publication Number:
  US 20020121314A1
• Time in Patent Office: 1,148 Days
• Field of Search: 435/6, 435/7.1, 435/91.1, 435/912, 435/283.2, 536/22.1, 536/23.7, 536/24-- 2, 204/193
• US Class Current: 506/39
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 40/00 Manufacture or treatment of nanostructures
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: G01N 2610/00 Assays involving self-assembled monolayers [SAMs]
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/553 Metal or metal coated

Abstract

The present invention relates to the use asymmetric monolayer forming species and electroconduit forming species to detect target analytes.

Claims

1. A composition comprising a metallic surface, said surface comprising:

a) a self-assembled monolayer (SAM) forming species comprising a capture probe, wherein said capture probe comprises a nucleic acid; and

,

b) an asymmetric monolayer forming species having the formula;

wherein

A is an attachment linker moiety selected from the group consisting of sulfur and phosphonate;

MFS is a monolayer forming species selected from the group consisting of conductive oligomers and insulators;

AG is an electroconduit forming species and,

wherein MFS and AG are different.

2. A composition according to claim 1 wherein A is sulfur.

3. A composition according to claim 1 wherein said metallic surface is gold.

4. A composition according to claim 1 wherein said MFS is an insulator.

5. A composition according to claim 4 wherein said insulator comprises an alkyl group from 7 to 20 carbons.

6. A composition according to claim 5 wherein said alkyl group comprises a heteroalkyl.

7. A composition according to claim 5 wherein said alkyl group comprises a substituted alkyl.

8. A composition according to claim 1 wherein said AG comprises an alkyl group from about 1 to 6 carbons.

9. A composition according to claim 1 or 8 wherein said AG is branched, having the formula:

wherein

R₁ through R₃ are each, independently from the others, selected from the group consisting of hydrogen, alkyl, aryl, alcohol, amine, amido, nitro, ether, ester, ketone, imino, aldehyde, alkoxy, carbonyl, halogen, sulfur containing moiety and phosphorus containing moiety.

10. A composition according to claim 9 wherein said AG is attached to said attachment linker via a (CH₂)_n group, wherein n is an integer from 0 to 4.

11. A composition according to claim 9 wherein said AG is attached directly to said attachment linker.

12. A composition according to claim 1, said asymmetric monolayer forming species having the formula:

13. A composition according to claim 1, wherein said SAM forming species is an insulator.

14. A composition according to claim 12, wherein said insulator comprises an alkyl group from 7 to 20 carbons.