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Buffer composition and method for hybridization of microarrays on adsorbed polymer siliceous surfaces

  • US 6,753,145 B2
  • Filed: 07/05/2001
  • Issued: 06/22/2004
  • Est. Priority Date: 07/05/2001
  • Status: Expired due to Fees
First Claim
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1. A method of hybridizing a microarray of oligonucleotides bound to a polymer adsorbed on a surface of a siliceous substrate with a nucleic acid material comprising the step ofincubating the nucleic acid material with the microarray of oligonucleotides on the adsorbed polymer surface in a hybridization solution at a hybridization temperature ranging from about 55°

  • C. to about 70°

    C. so as to hybridize the nucleic acid material, wherein the hybridization solution comprises a buffer composition that comprises a pH within a range of pH 6.4 to 7.5, a non-chelating buffering agent that maintains the pH within the pH range, and a monovalent cation in a monovalent cation concentration ranging from about 0.01 M to about 2.0 M.

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