P-glycoproteins and uses thereof

• US 6,753,177 B1
• Filed: 09/28/2000
• Issued: 06/22/2004
• Est. Priority Date: 09/28/1999
• Status: Expired due to Fees

• Current Assignee: Corning Incorporated
• Original Assignee: Becton, Dickinson & Co
• Inventors: Crespi, Charles L., Patten, Christopher J., Stocker, Penny J., Steimel-Crespi, Dorothy T., Reif, Timothy C.
• Primary Examiner(s): RAO, MANJUNATH N
• Application Number:
  US09/672,725
• Time in Patent Office: 1,363 Days
• Field of Search: 435/69.1, 435/183, 435/252.3, 435/320.1, 536/23-- 2, 530/350, 436/87
• US Class Current: 435/252.3
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748

Abstract

The invention pertains to dog P-glycoproteins and related P-glycoproteins which include dog-specific amino acids, as well as nucleic acids which encode those polypeptides. The present invention also includes fragments and biologically functional variants of the dog P-glycoprotein. The invention further relates to methods of using such dog P-glycoprotein nucleic acids and polypeptides, especially in methods for determining bioavailability of drugs and for screening for inhibitors of dog PGP. Also included are dog PGP inhibitors which inhibit dog PGP activity by inhibiting the expression or function of dog PGP.

Claims

1. An isolated nucleic acid molecule that codes for the amino acid sequence of SEQ ID NO:

2 and complements thereof.

4. An expression vector comprising the isolated nucleic acid molecule of claim 1 operably linked to a promoter.

5. A host cell transformed or transfected with the expression vector of claim 4.

2. An isolated nucleic acid molecule that codes for the amino acid sequence of SEQ ID NO:

2, wherein the isolated nucleic acid comprises the nucleotide sequence of SEQ ID NO;

1.

3. An isolated nucleic acid molecule that codes for the amino acid sequence of SEQ ID NO:

2, wherein the isolated nucleic acid comprises the coding region of SEQ ID NO;

1.