Method for producing template-textured materials with high binding specificity and selectivity and utilization of said materials

• US 6,753,396 B2
• Filed: 09/03/2002
• Issued: 06/22/2004
• Est. Priority Date: 12/03/1999
• Status: Expired due to Fees

• Current Assignee: PolyAn Gesellschaft zur Herstellung von Polymeren für spezielle Anwendungen und Analytik mbH
• Original Assignee: Elipsa GmbH
• Inventors: Piletsky, Sergey A., Sergeyeva, Tatinna, Matuschewski, Heike, Ulbricht, Mathias, Schedler, Uwe
• Primary Examiner(s): Choi, Ling-Siu
• Application Number:
  US10/148,819
• Publication Number:
  US 20030130462A1
• Time in Patent Office: 658 Days
• Field of Search: 526/348, 526/346, 526/317.1, 526/303.1, 526/242, 526/274
• US Class Current: 526/317.1
• CPC Class Codes: B01J 20/268 Polymers created by use of a template, e.g. molecularly imprinted polymers
• CPC Class Codes: B01J 20/281 Sorbents specially adapted for preparative, analytical or investigative chromatography
• CPC Class Codes: B01J 20/285 based on polymers
• CPC Class Codes: B01J 20/3057 Use of a templating or imprinting material molecularly imprinted polymers B01J20/268; filling pores of a substrate or matrix followed by the removal of the substrate or matrix
• CPC Class Codes: B01J 20/3204 Inorganic carriers, supports or substrates
• CPC Class Codes: B01J 20/321 consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3212 consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/327 Polymers obtained by reactions involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3272 Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3274 Proteins, nucleic acids, polysaccharides, antibodies or antigens
• CPC Class Codes: B01J 20/3276 Copolymers
• CPC Class Codes: B01J 20/3278 Polymers being grafted on the carrier
• CPC Class Codes: B01J 20/3282 Crosslinked polymers
• CPC Class Codes: B01J 20/3285 Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic
• CPC Class Codes: B01J 2220/54 Sorbents specially adapted for analytical or investigative chromatography

Abstract

The invention relates to a method for the production of a template-textured material by synthesis of a template-textured polymer (TTP) by performing crosslinking polymerization of functional monomers on a support in the presence of a template, which method is characterized in that a support having a thin polymer layer on the surface thereof is added with a reaction mixture consisting of polymerization initiator, template, functional monomer, crosslinker, solvent and/or buffer and, following sorption of the reaction mixture in the thin polymer layer, the polymerization is initiated and continued until the absorption capacity of the thin polymer layer for the template-textured polymer (TTP) is reached, and the template is optionally removed in a final step, the support used being selected in such a way that it cannot absorb the reaction solution. The materials of the invention are remarkable for their high binding specificity and selectivity for the template.

Claims

1. A method of producing a template-textured material by crosslinking polymerization of functional monomers in the presence of a template on a support, wherein the method comprises,

providing a two-layered support, the first layer comprising a solid supporting material, and the second layer comprising a thin polymer layer on the surface of the first layer;

contacting the two-layered support with a reaction mixture comprising polymerization initiator, template, molecule, functional monomer, crosslinking agent, solvent and/or buffer, wherein the contacting is performed for period of time to affectively permit sorption of the reaction mixture into the thin polymer;

initiating the polymerization and continuing the polymerization until the maximum extent of absorption of the thin polymer layer is reached,

and, optionally, removing the template,

wherein the solid supporting material is non-absorbant with respect to the reaction mixture.

2. The method according to claim 1, wherein

the reaction mixture is added in such a way that initially, the major portion of the polymerization initiator in a solvent is added and subsequently, the reaction mixture comprised of initiator residual amount, template, functional monomer, crosslinker, solvent and/or buffer is added.

3. The method according to claim 1, wherein

the reaction mixture is added in such a way that initially, the entire polymerization initiator in a solvent is added and subsequently, the residual reaction mixture comprised of template, functional monomer, crosslinker, solvent and/or buffer is added.

4. The method according to claim 1, wherein

a molded piece made of a hydrophobic material is used as support, and a layer of a hydrophilic polymer is used as thin polymer layer.

5. The method according to claim 1, wherein

an inorganic solid is used as support, and a layer of a hydrophilic or hydrophobic polymer is used as thin polymer layer.

6. The method according to claim 1, wherein

a molded piece comprised of a highly crosslinked organic polymer is used as support, and a layer of a hydrophilic or hydrophobic polymer is used as thin polymer layer.

7. The method according to any of claim 4, 5 or 6, wherein

films, foils, plates, particularly microtiter plates, reaction vessels of any shape, particles, particularly micro- or nanoparticles, fibers, particularly hollow fibers, fabrics, fleeces, filters, or membranes made of inorganic or organic materials are used as support.

8. The method according to claim 4, wherein

hydrophobic organic polymers are used as hydrophobic support materials, preferably polypropylene, polyethylene, polystyrene, polysulfone, hydrophobic polyamides, hydrophobic polyesters, polycarbonate, polyacrylonitrile, poly(vinylidene fluoride), polytetrafluoroethylene, hydrophobic polyacrylates, as well as derivatives, copolymers or blends of these polymers.

9. The method according to claim 5, wherein

glasses, silicates, ceramics, or metals or composites thereof, also including hydrophobic or crosslinked organic polymers, are used as inorganic solids.

10. The method according to claim 6, wherein

highly crosslinked polystyrene and polystyrene derivatives or copolymers, or highly crosslinked polyacrylates are used as highly crosslinked organic polymer.

11. The method according to claim 7, wherein

a porous membrane having a pore size between 2 nm and 10 μ

m, preferably from 100 nm to 5 μ

m, is used as support.

12. The method according to claims 4, wherein

a layer of crosslinked or non-crosslinked hydrophilic polyacrylates, polyacrylamides, cellulose, amylose, agarose, as well as derivatives, copolymers or blends thereof, particularly of 2-hydroxypropyl methacrylate crosslinked with tetraethylene glycol bismethacrylate, is used as thin hydrophilic polymer layer.

13. The method according to claim 5 or 6, wherein

a layer of crosslinked or non-crosslinked fluorinated polymers, silicones, paraffins, or waxes, as well as derivatives, copolymers or blends thereof is used as thin hydrophobic polymer layer.

14. The method according to claim 1, wherein

the thickness of the polymer layer on the support is from 1 nm to 1 μ

m, preferably about 5 to 20 nm.

15. The method according to claim 4, wherein

a hydrophilized poly(vinylidene fluoride) membrane is used as hydrophobic support having a thin hydrophilic polymer layer on the surface thereof.

16. The method according to claim 1, wherein

the polymerization is conducted as a photoinitiated crosslinking graft copolymerization of the functional monomers, using an H abstraction type substance as photoinitiator and the thin polymer layer as co-initiator.

17. The method according to claim 1, wherein

small molecules having a molecular mass of up to 100 Da, larger molecules of up to 1,000,000 Da, or even microorganisms or cells are used as templates.

18. The method according to claim 1, wherein

polymerizable compounds including groups capable of interacting with templates, particularly carboxyl, sulfonyl, sulfate, phosphate, amino, or quaternary ammonium groups, or derivatives thereof, also in admixture, preferably AMPS, MAA or AA, are used as functional monomers.

19. A template-textured material, produced according to the method as claimed in claims 1.