Human secretory type phospholipase a2

• US 6,756,219 B1
• Filed: 06/11/2001
• Issued: 06/29/2004
• Est. Priority Date: 12/09/1998
• Status: Expired due to Fees

• Current Assignee: Shionogi & Co., Ltd.
• Original Assignee: Shionogi & Co., Ltd.
• Inventors: Suzuki, Noriko, Ishizaki, Jun, Hanasaki, Kohji
• Primary Examiner(s): Achutamurthy, Ponnathapu
• Assistant Examiner(s): PAK, YONG D
• Application Number:
  US09/856,486
• Time in Patent Office: 1,114 Days
• Field of Search: 435/197, 435/440, 536/23.2, 424/94.6
• US Class Current: 435/197
• CPC Class Codes: C12N 9/16 acting on ester bonds (3.1)
• CPC Class Codes: C12N 9/18 Carboxylic ester hydrolases (3.1.1)
• CPC Class Codes: C12Q 1/34 involving hydrolase
• CPC Class Codes: G01N 33/573 for enzymes or isoenzymes

Abstract

This invention relates to a gene encoding human secretory type phospholipase A₂ (PLA₂). According to the invention, an expression vector having this gene, and a transformant having the expression vector are obtainable. The PLA₂ protein can be produced by the culture of the transformant.

Claims

1. An isolated polypeptide that has phospholipase A2 activity that is encoded by a nucleic acid sequence that hybridizes to the complement of SEQ ID NO:

26 under stringent conditions, wherein stringent conditions comprise heating at 42°

C. in 6×

SSC, 0.5% SDS and 50% formamide and washing at 68°

C. in 0.1×

SSC and 0.5% SDS.

4. The polypeptide of claim 1 that is a fragment of SEQ ID NO:

27.

5. The polypeptide of claim 1 that comprises amino acid residues 1 to 125 of SEQ ID NO:

27.

6. The polypeptide of claim 1 that comprises residues −

20 to 125 of SEQ ID NO;

27.

7. The polypeptide of claim 1 that is a recombinant polypeptide expressed in a prokaryotic cell.

8. The polypeptide of claim 1 that is a recombinant polypeptide expressed in a eukaryotic cell.

9. A composition comprising the polypeptide of claim 1.

10. A composition comprising the polypeptide of claim 1 and a substrate, wherein said polypeptide is immobilized on said substrate.

11. The composition of claim 10, wherein said substrate is an insoluble polysaccharide.

12. The composition of claim 10, wherein said substrate is a synthetic resin.

13. The composition of claim 10, wherein said substrate is glass.

14. The composition of claim 10, wherein said substrate is silicon.

15. A diagnostic reagent comprising the polypeptide of claim 1.

16. An assay kit comprising the polypeptide of claim 1.

2. An isolated polypeptide that has phospholipase A2 activity that comprises SEQ ID NO:

27.

3. The polypeptide of claim 2 consisting of SEQ ID NO:

27.