Device for receiving a chip shaped carrier and process for assembling a plurality of such devices

• US 6,756,224 B2
• Filed: 07/26/2002
• Issued: 06/29/2004
• Est. Priority Date: 07/30/2001
• Status: Expired due to Term

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Roche Molecular Systems (Roche Holding AG)
• Inventors: Vischer, Peter
• Primary Examiner(s): Redding, David A.
• Application Number:
  US10/205,734
• Publication Number:
  US 20030064506A1
• Time in Patent Office: 704 Days
• Field of Search: 435/287.2, 435/288.3, 435/288.7, 435/809, 422/102, 422/104, 156/293, 156/294
• US Class Current: 506/9
• CPC Class Codes: B01J 2219/00274 Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00707 separated from the reactor apparatus
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0809 rectangular shaped
• CPC Class Codes: B01L 2400/0677 phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: B01L 9/52 Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: H01L 21/67121 Apparatus for making assemblies not otherwise provided for, e.g. package constructions
• CPC Class Codes: H01L 21/67144 Apparatus for mounting on conductive members, e.g. leadframes or conductors on insulating substrates
• CPC Class Codes: Y10S 435/809 Incubators or racks or holders for culture plates or containers

Abstract

A device for receiving a chip shaped carrier having an active surface, e.g. a surface which carries an array of DNA oligonucleids and which is adapted to be read by an electro-optical reading device. The device comprises: a cartridge having an opening for receiving a liquid sample and including casing parts (14, 15); a casing part having an inner surface and outer surface, a first cavity for receiving a chip shaped carrier, and means which provide access to that first cavity and thereby to the active surface of the chip shaped carrier; a sealing material which is at least once reversibly liquidifiable; the shape and dimensions of first cavity, chip shaped carrier, and sealing material, being chosen that the chip shaped carrier fits into the space delimited by the sealing material. The process for mounting the carrier in the cartridge essentially consists in placing the carrier in the first cavity and liquidifying the sealing material. In the liquid state, the sealing material closes any gap between the side walls of the first cavity and the carrier, and after solidification, the sealing material constitutes a strong and liquid-tight bond.

Claims

1. A device for receiving a chip shaped carrier having on one side a first surface which includes an active surface coated with an array of DNA snippets or the like, a second surface on a second side opposite to said first side, and an edge having a peripheral outer surface which extends between said first and said second surface, said active surface being adapted to be read by an electro-optical reading device, said device comprising

(a) a cartridge having an opening for introducing a liquid sample into said cartridge,

(b) said cartridge having a casing part which has an inner surface and an outer surface, a first cavity for receiving a chip shaped carrier,

(c) a layer of a sealing material which is reversibly transformable at least once from a solid state into a fluid state and which is provided in a sufficient amount to establish a liquid-tight bond between the inner surface of side walls of the first cavity and said peripheral outer surface of the edge of the chip shaped carrier when the latter is placed into said first cavity.

2. The chip receiving device according to claim 1,

wherein the sealing material liquidifies when heated over a certain temperature.

3. The chip receiving device according to claim 2,

wherein the sealing material liquidifies when heated by irradiation of energy.

4. The chip receiving device according to claim 1, wherein the sealing material is chosen such that it does not interfere with the optical observation method performable by an electro-optical reading device.

5. The chip receiving device according to claim 1, wherein said layer of sealing material extends on and over the entire inner periphery of the inner surface of the side walls of the first cavity.

6. The chip receiving device according to claim 1, wherein the sealing material constitutes a limitation of the first cavity tapering towards the bottom of it in order to facilitate inserting the chip shaped carrier into the first cavity.

7. The chip receiving device according to claim 1, wherein the bottom of the first cavity is provided with an opening so that the active surface of a chip placed in the first cavity is accessible for optical examination.

8. An analytical cartridge comprising a chip shaped carrier inserted in the first cavity of a chip receiving device according to claim 1, wherein the sealing material fills the clearance between the walls of the first cavity and the chip.

9. The analytical cartridge according to claim 8, wherein the bond established by the sealing material withstands a pressure of at least 300 mbar, preferably at least 500 mbar, at 20°

C. for at least one hour on the chip shaped carrier.

10. The analytical cartridge according to claim 8, wherein the means providing access to an active area of the chip shaped carrier is an opening in the bottom of the first cavity, said opening having an inner end and an outer end, and wherein the periphery of said inner end of said opening surrounds the active surface.

11. A process for assembling a plurality of analytical cartridges according to claim 8 comprising:

(a) providing a plurality of casing parts to an automated assembly line,

(b) providing a plurality of chip shaped carriers having each an active surface to said assembly line,

(c) positioning each of said chip shaped carriers within the first cavity of one of said casing parts by means of a first automatic apparatus,

(d) bonding each of said chip shaped carriers in said first cavity of said casing part, by liquidifying the sealing material.

12. The process according to claim 11, wherein the liquidifying of the sealing material is performed by irradiating said sealing material with an energy form.

13. The process according to claim 12, wherein the sealing material is irradiated with laser light.

14. The process according to claim 12, wherein the sealing material is irradiated with ultrasonic waves.

15. The process according to claim 11, wherein all assembling steps defined therein are fully automated.

16. The process according to claim 11, wherein the following steps are carried out in a clean room:

positioning each of said carriers within a first cavity of one of said casing parts, and

bonding of the carrier to the casing part by liquidifying the sealing material.

17. A system for carrying out evaluation and/or monitoring methods involving electro-optical reading of an active surface of a chip shaped carrier, said system comprising

(a) a cartridge having an opening for introducing a liquid sample into said cartridge, and a casing part,

(a.1) said casing part being comprised in the cartridge and having an outer surface and an inner surface, a first cavity for receiving a chip shaped carrier, and means which provide access to a first cavity and thereby to said active surface of said chip shaped carrier, and

(a.2) said first cavity having a flat bottom surface and side wall surfaces which extend between said outer surface of said casing part and said bottom surface;

(b) a sealing material being provided in said first cavity, said sealing material consisting of an at least once reversibly liquidifiable material, and

(c) a chip shaped carrier having an active surface which is adapted to be read by an electro-optical reading device, said chip shaped carrier being positioned in said first cavity of said casing part, and the sealing material filling the gap between the cavity walls, particularly its side walls, and the chip shaped carrier in order to hold the chip shaped carrier firmly and fluid-tightly in the cavity.

18. The system according to claim 17, said system further comprising means for electro-optically reading said active surface of said chip shaped carrier.