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Culturing pancreatic stem cells having a specified, intermediate stage of development

  • US 6,759,039 B2
  • Filed: 06/29/2001
  • Issued: 07/06/2004
  • Est. Priority Date: 06/30/2000
  • Status: Expired due to Term
First Claim
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1. A method of producing an aggregate of cultured pancreatic cells that comprises an encapsulating mantle of ck-19 positive cells and an inner cell mass, wherein the aggregate comprises 50-5000 pancreatic cells and has a diameter of between 50 and 300 microns, the method comprising the steps of(a) culturing pancreatic cells on a substrate;

  • (b) removing the cells from the substrate;

    (c) reseeding PDX-1 positive pancreatic cells on a substrate produced by step (b); and

    (d) culturing the cells of step (c) on the substrate of step (b) to provide an aggregate of cultured pancreatic cells that comprise a surrounding mantle of ck-19 positive cells and an inner cell mass, wherein the aggregate comprises 50-5000 pancreatic cells and has a diameter of between 50 and 300 microns.

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