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Enzymes for the detection of specific nucleic acid sequences

  • US 6,759,226 B1
  • Filed: 05/24/2000
  • Issued: 07/06/2004
  • Est. Priority Date: 05/24/2000
  • Status: Expired due to Term
First Claim
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1. A composition comprising a polymerase comprising a 5′

  • nuclease, wherein said polymerase comprises a heterologous functional domain, wherein said heterologous functional domain comprises an amino acid sequence that provides improved background specificity in a nucleic acid cleavage assay compared to said polymerase enzyme without said heterologous functional domain.

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