Alkaline protease

• US 6,759,228 B2
• Filed: 08/03/2001
• Issued: 07/06/2004
• Est. Priority Date: 10/07/1997
• Status: Expired due to Term

• Current Assignee: KAO Corporation
• Original Assignee: KAO Corporation
• Inventors: Nomura, Masafumi, Kubota, Hiromi, Saeki, Katsuhisa, Kageyama, Yasushi, Okuda, Mitsuyoshi, Shikata, Shitsuw, Hitomi, Jun, Takaiwa, Mikio
• Primary Examiner(s): RAO, MANJUNATH N
• Application Number:
  US09/920,954
• Publication Number:
  US 20020064854A1
• Time in Patent Office: 1,068 Days
• Field of Search: 435/4, 435/6, 435/69.1, 435/183, 435/195, 435/212-229, 510/114, 510/392, 510/515
• US Class Current: 435/220
• CPC Class Codes: C11D 3/386 Preparations containing enzymes , e.g. protease or amylase
• CPC Class Codes: C11D 3/38609 Protease or amylase in solid compositions only
• CPC Class Codes: C11D 3/38618 Protease or amylase in liquid compositions only
• CPC Class Codes: C12N 9/54 bacteria being Bacillus

Abstract

An alkaline protease having the following properties; a gene encoding the same; a microorganism producing the same; and washing compositions containing the same; (i) acting over a broad pH value range of 4 to 13 and achieving, at pH 6 to 12, 80% or more the activity at the optimum pH value; (ii) when treated at 40° C. for 30 minutes, being stable over a pH value range of 6 to 11; (iii) having an isoelectric point of about 8.9 to 9.1; and (iv) having casein digesting activity that is not inhibited by oleic acid. The alkaline protease of the present invention is highly stable to various surface active agents and fatty acids, and exhibits high stability to oxidizing agents, and is therefore useful as an enzyme to be used in detergents for automatic dishwashers and laundry detergents, both containing bleaching components.

Claims

1. An isolated alkaline protease having an amino acid sequence which is at least 90% homologous to an amino acid sequence selected from the group consisting of SEQ ID NO:

1 and SEQ ID NO;

2, wherein said isolated alkaline protease has alkaline protease activity.

2. The alkaline protease of claim 1, which has an amino acid sequence that is at least 90% homologous to the amino acid sequence of SEQ ID NO:

1.

3. A detergent composition comprising the alkaline protease of claim 2.

4. The detergent composition of claim 3, which contains 0.1 to 5000 U of the alkaline protease per kg of the composition.

5. The detergent composition of claim 4, which comprises a surfactant.

6. The detergent composition of claim 4, which comprises 0.5 to 60 wt. % of the detergent.

7. The detergent composition of claim 4, which contains at least one enzyme other than the alkaline protease.

8. The alkaline protease of claim 1, which has an amino acid sequence that is at least 90% homologous to the amino acid sequence of SEQ ID NO:

2.

9. A detergent composition comprising the alkaline protease of claim 8.

10. The detergent composition of claim 9, which contains 0.1 to 5000 U of the alkaline protease per kg of the composition.

11. The detergent composition of claim 10, which comprises a surfactant.

12. The detergent composition of claim 10, which comprises 0.5 to 60 wt. % of the detergent.

13. The detergent composition of claim 10, which contains at least one enzyme other than the alkaline protease.

14. The alkaline protease of claim 1, wherein said alkaline protease has the following physicochemical properties:

(i) Acting pH range acting over a wide pH range of 4-13 and exhibiting, at a pH of 6-12, 80% or more of the activity at the optimum pH;

(ii) Stable pH range being stable over a pH range of 6-11 when treated at 40°

C. for 30 minutes;

(iii) Isoelectric point having an isoelectric point of approximately 8.9-9.1; and

(iv) Effect of a fatty acid casein-degrading activity not being inhibited by oleic acid.