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Composite blastocysts (CBs) from aggregates of dissociated cells of non-viable pre-embryos

  • US 6,759,244 B2
  • Filed: 11/08/2001
  • Issued: 07/06/2004
  • Est. Priority Date: 11/08/2001
  • Status: Expired due to Fees
First Claim
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1. A method of producing composite blastocysts comprising:

  • a) dissociation of non-viable mammalian pre-blastocyst embryos into non-nucleated and individual nucleated cells or groups of cells;

    b) isolation of individual mononucleated cells or groups of mononucleated cells from disaggregated non-viable pre-blastocyst embryos;

    c) aggregation of isolated mononucleated cells or groups of mononucleated cells from non-viable pre-blastocyst embryos in a host zona pellucida; and

    d) culturing of the zona-encapsulated cell aggregates to allow multiplication of cells to form a composite blastocyst.

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