Printed circuit boards with monolayers and capture ligands

• US 6,761,816 B1
• Filed: 03/08/2000
• Issued: 07/13/2004
• Est. Priority Date: 06/23/1998
• Status: Expired due to Term

• Current Assignee: Clinical Micro Sensors Inc. (Roche Holding AG)
• Original Assignee: Clinical Microsystems Intl.
• Inventors: Vielmetter, Jost G., Creager, Stephen E., Welch, Thomas W., Meade, Thomas J., Terbrueggen, Robert H., Blackburn, Gary, Irvine, Bruce D., Fraser, Scott, OʼConnor, Stephen D.
• Primary Examiner(s): Nguyen, Nam
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/520,477
• Time in Patent Office: 1,588 Days
• Field of Search: 204/400, 204/403, 204/411, 204/412, 204/416, 204/418, 204/290.R, 204/403.01, 204/403-6-4, 205/777.5
• US Class Current: 205/777.5
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2565/629 being a microfluidic device

Abstract

The invention relates to compositions and methods useful in the acceleration of binding of target analytes to capture ligands on surfaces. Detection proceeds through the use of an electron transfer moiety (ETM) that is associated with the target analyte, either directly or indirectly, to allow electronic detection of the ETM.

Claims

1. A device comprising:

a) a cartridge comprising a substrate comprising a printed circuit board, said printed circuit board comprising a plurality of gold electrodes each comprising;

i) a self-assembled monolayer;

ii) a capture ligand; and

,

iii) an interconnect such that each electrode is independently addressable; and

b) a detector for detecting an individual output signal from each of said electrodes.

3. A device according to claim 1 or 2 wherein said printed circuit board material is fiberglass.

4. A device according to claim 1 or 2 wherein said printed circuit board is GETEK®

.

5. A device according to claim 1 or 2 wherein said printed circuit board comprises a plurality of layers.

6. A device according to claim 1 or 2 wherein said cartridge further comprises a sample detection chamber.

7. A device according to claim 1 or 2 wherein said self-assembled monolayer further comprises insulators.

8. A device according to claim 7 wherein said insulators comprise alkyl groups.

9. A device according to claim 7 wherein said insulators comprise terminal groups.

10. A device according to claim 9 wherein said terminal groups of said insulators comprise ethylene glycol.

11. A device according to claim 1 or 2 wherein said self-assembled monolayer comprises a conductive oligomer.

12. A device according to claim 1 or 2 wherein said self-assembled monolayer comprises a mixture of conductive oligomers and insulators.

13. A device according to claim 1 or 2 wherein said capture ligand is covalently attached to an electrode via an attachment linker.

14. A device according to claim 13 wherein said attachment linker is an insulator.

15. A device according to claim 1 or 2 wherein said capture ligand is a nucleic acid.

16. A device according to claim 1 or 2 wherein said capture ligand is a protein.

2. A device comprising:

a) a cartridge comprising a substrate comprising a printed circuit board, said printed circuit board comprising a plurality of gold electrodes each comprising;

i) a self-assembled monolayer attached to said electrode by thiol linkages;

ii) a capture ligand; and

,

iii) an interconnect such that each electrode is independently addressable;

b) an AC/DC voltage source; and

c) a detector for detecting an individual output signal from each of said electrodes.

17. A method of detecting a target analyte in a sample, comprising:

a) placing said sample in a cartridge, said cartridge comprising a substrate comprising a printed circuit board, wherein said printed circuit board comprises a plurality of electrodes each comprising;

i) a self-assembled monolayer;

ii) a capture ligand; and

b) a detector for detecting an individual output signal from each of said electrodes.

19. A method according to claim 17 or 18, wherein said self-assembled monolayer is attached to said electrode by a thiol linkage.

20. A method according to claim 17 or 18 wherein said printed circuit board material is fiberglass.

21. A method according to claim 17 or 18 wherein said printed circuit board is GETEK®

.

22. A method according to claim 17 or 18 wherein said printed circuit board comprises a plurality of layers.

23. A method according to claim 17 or 18 wherein said cartridge further comprises a sample detection chamber.

24. A method according to claim 23 wherein said insulators comprise alkyl groups.

25. A method according to claim 24 wherein said terminal groups of said insulators comprise ethylene glycol.

26. A method according to claim 23 wherein said insulators comprise terminal groups.

27. A method according to claim 17 or 18 wherein said self-assembled monolayer further comprises insulators.

28. A method according to claim 17 or 18 wherein said self-assembled monolayer comprises a conductive oligomer.

29. A method according to claim 17 or 18 wherein said self-assembled monolayer comprises a mixture of conductive oligomers and insulators.

30. A method according to claim 29 wherein said attachment linker is an insulator.

31. A method according to claim 17 or 18 wherein said capture ligand is covalently attached to an electrode via an attachment linker.

32. A method according to claim 17 or 18 wherein said capture ligand is a nucleic acid.

33. A method according to claim 17 or 18 wherein said capture ligand is a protein.

18. A method of detecting a target analyte in a sample, comprising:

a) providing a cartridge comprising a substrate comprising a printed circuit board, wherein said printed circuit board comprises a plurality of electrodes each comprising;

i) a self-assembled monolayer;

ii) a capture ligand; and

iii) an interconnect such that each electrode is independently addressable;

b) adding said sample to said cartridge such that said target analyte and said capture ligand bind; and

,

c) detecting an individual output signal from each of said electrodes.

34. A method according to claim 18, wherein said sample comprises a plurality of different target analytes and each of said target analytes binds to a different capture ligand.