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Compositions and methods for analysis of nucleic acids

  • US 6,762,022 B2
  • Filed: 03/06/2001
  • Issued: 07/13/2004
  • Est. Priority Date: 03/05/1997
  • Status: Expired due to Term
First Claim
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1. A method for preparing a DNA molecule comprising the steps of:

  • a) obtaining a sample of DNA wherein the sample includes DNA fragments that do not include a 3′

    hydroxyl group;

    b) conditioning DNA fragments of the sample to provide a 3′

    hydroxyl group thereon; and

    c) attaching a double stranded oligonucleotide adaptor to only one strand of the conditioned DNA fragment.

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