Micromachined device for receiving and retaining at least one liquid droplet, method of making the device and method of using the device

• US 6,764,652 B2
• Filed: 01/24/2001
• Issued: 07/20/2004
• Est. Priority Date: 01/24/2001
• Status: Expired due to Term

• Current Assignee: Board of Regents of the University of Michigan (University of Michigan)
• Original Assignee: Board of Regents of the University of Michigan (University of Michigan)
• Inventors: Brown, Richard B., Hower, Robert W.
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/768,950
• Publication Number:
  US 20040071598A1
• Time in Patent Office: 1,273 Days
• Field of Search: 436/174, 436/178, 204/403.4, 204/403.05, 204/403.13, 204/415, 204/418, 204/416, 422/52, 422/57, 422/58, 422/102, 422/99
• US Class Current: 422/552
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 2035/1037 Using surface tension, e.g. pins or wires
• CPC Class Codes: Y10T 436/25 including sample preparation

Abstract

A micromachined device such as a solid-state liquid chemical sensor for receiving and retaining a plurality of separate liquid droplets at desired sites, a method of making the device and a method of using the device are provided. The technique works for both aqueous and solvent-based solutions. The device includes a substrate having an upper surface, and a first set of three-dimensional, thin film well rings patterned at the upper surface of the substrate. Each of the wells is capable of receiving and retaining a known quantity of liquid at one of the desired sites through surface tension. A method for patterning a membrane/solvent solution results in reproducibly-sized, uniformly-thick membranes. The patterning precision of this method allows one to place the membranes closer together, making the sensors smaller and less expensive, and the uniform film thickness imparts reproducibility to the sensors.

Claims

1. A micromachined device comprising:

a substrate having an upper surface; and

an array of three-dimensional, thin film wells patterned at the upper surface of the substrate wherein each of the wells is capable of receiving and retaining a know quantity of liquid;

wherein each of the wells includes a side wall having an outside corner with a small radius to prevent the liquid from flowing down outside the side wall.

2. A method of making a micromachined device comprising;

providing a substrate having a layer of radiation-sensitive material formed thereon;

patterning at least one three-dimensional, thin film well from the layer of material wherein the at least one well is capable of receiving and retaining a known quantity of liquid; and

dispensing a first membrane solution into the well wherein the membrane is an optical membrane.

3. A method of making a micromachined device comprising:

providing a substrate having a layer of radiation-sensitive material formed thereon;

patterning at least one three-dimensional, thin film well from the layer of material wherein the at least one well is capable of receiving and retaining a known quantity of liquid;

dispensing a first membrane solution into the well;

patterning a three-dimensional, thin film well from the layer of material outside of at least one well at the same time as patterning the at least one well,

dispensing a second membrane solution over the first membrane solution and into the thin film well outside the at least one well.

4. The method as claimed in claim 3 wherein the first membrane solution is an internal filling solution.

5. The method as claimed in claim 3 wherein the second membrane solution is an external binding layer.

6. The method as claimed in claim 3 wherein the second membrane solution has ionophores, enzymes, antibodies or functional groups trapped therein.