Thermomyces lanuginosus kinesin motor protein and methods of screening for modulators of kinesin proteins

• US 6,764,830 B1
• Filed: 12/21/2000
• Issued: 07/20/2004
• Est. Priority Date: 01/23/1998
• Status: Expired due to Fees

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Goldstein, Lawrence S. B., Sakowicz, Roman
• Primary Examiner(s): Prouty, Rebecca
• Assistant Examiner(s): Steadman, David J
• Application Number:
  US09/600,823
• Time in Patent Office: 1,307 Days
• Field of Search: 435/196, 435/19, 530/330
• US Class Current: 435/19
• CPC Class Codes: C07K 14/37 from fungi

Abstract

The invention provides isolated nucleic acid and amino acid sequences of TL-_γ, antibodies to TL-_γ, methods of screening for TL-_γ modulating using biologically active TL-_γ, and kits for screening for TL-_γmodulators.

Claims

1. An isolated protein comprising amino acid residues 1 to 357 of SEQ ID NO:

1, wherein said isolated protein has at least one activity selected from the group consisting of plus-end directed microtubule motor activity, microtubule binding activity, and ATPase activity.

2. The isolated protein of claim 1, wherein the protein specifically binds to polyclonal antibodies to SEQ ID NO:

1.

3. The isolated protein of claim 1, wherein the protein specifically binds to polyclonal antibodies generated against amino acid residues 1 to 357 of SEQ ID NO:

1.

5. A kit for screening for modulators of Thermomyces lanuginosus gamma kinesin protein activity, wherein said kit comprises:

(i) a container holding an isolated protein of claim 1, and

(ii) instructions for assaying for at least one Thermomyces lanuginosus gamma kinesin protein activity, wherein the Thermomyces lanuginosus gamma kinesin protein activity is selected from the group consisting of plus end-directed microtubule motor activity, binding activity, and ATPase activity.

6. The kit of claim 5, wherein the protein further comprises amino acid residues 443 to 601 of SEQ ID NO:

1.

7. The kit of claim 5, wherein the protein is recombinant.

8. The isolated protein of claim 1, wherein the protein further comprises amino acids 443 to 601 of SEQ ID NO:

1.

10. The isolated protein of claim 1, wherein said isolated protein comprises SEQ ID NO:

1.

11. The kit of claim 5, wherein said isolated protein comprises SEQ ID NO:

1.

4. An isolated protein consisting of amino acid residues 1-357 of SEQ ID NO:

1, wherein said isolated protein has at least one activity selected from the group consisting of plus-end directed microtubule motor activity, microtubule binding activity, and ATPase activity.

9. A kit for screening for modulators of Thermomyces lanuginosus gamma kinesin protein activity, wherein said kit comprises:

(i) a container holding an isolated protein of claim 4; and

(ii) instructions for assaying for at least one Thermomyces lanuginosus gamma kinesin protein activity, wherein the Thermomyces lanuginosus gamma kinesin protein activity is selected from the group consisting of plus end-directed microtubule motor activity, binding activity, and ATPase activity.