Xylanase from trichoderma reesei, method for production thereof, and methods employing this enzyme

• US 6,768,001 B2
• Filed: 05/31/2002
• Issued: 07/27/2004
• Est. Priority Date: 12/30/1999
• Status: Expired due to Term

• Current Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Original Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Inventors: Saloheimo, Markku L A, Siika-Aho, Matti, Penttila, Merja E., Tenkanen, Maija
• Primary Examiner(s): Ketter, James
• Assistant Examiner(s): Lambertson, David A.
• Application Number:
  US10/159,487
• Publication Number:
  US 20030054518A1
• Time in Patent Office: 788 Days
• Field of Search: 435/4, 435/6, 435/41, 435/69.1, 435/71.1, 435/440, 435/471, 435/476, 435/243, 435/254.1, 435/254.11, 435/320.1, 536/23.1, 536/23.74
• US Class Current: 536/23.1
• CPC Class Codes: C12N 9/248 Xylanases

Abstract

The present invention is directed to novel xylanases (referred to as XYL-IV) and to nucleic acid molecules encoding those xylanases. Also provided herein are vectors and host cells including those nucleic acid sequences, antibodies which bind to the xylanases of the present invention, methods for producing the xylanases of the present invention, and methods employing the xylanases of the present invention.

Claims

1. An isolated nucleic acid molecule comprising DNA having at least 90% sequence identity to the nucleotide sequence of SEQ ID NO:

1 wherein said isolated nucleic acid molecule encodes a XYL-IV protein having the sequence of SEQ ID NO;

2.

2. An isolated nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:

1.

3. An isolated DNA encoding a XYL-IV protein having the amino acid sequence of SEQ ID NO:

2.

4. The DNA of claim 3, wherein the DNA Is derived from a fungus or bacteria.

5. The DNA of claim 4, wherein the fungus comprises a filamentous fungus.

6. The DNA of claim 5, wherein the filamentous fungus is selected from the group consisting of Trichoderma spp., Humicola spp., Neurospora spp., aspergillus spp., Fusarium spp., Penicillium spp., and Gliocladium spp.

7. The DNA of claim 6, wherein the filamentous fungus comprises Trichoderma reesei.

8. An isolated nucleic molecule comprising the complement of the nucleotide sequence of SEQ ID NO:

1.

9. A vector comprising a nucleic acid molecule encoding the polypeptide illustrated in SEQ ID NO:

2.

10. A host cell transformed with the vector of claim 9.

11. The host cell of claim 10, wherein the host cell is a filamentous fungus.

12. A vector comprising an isolated DNA comprising the nucleotide sequence of SEQ ID No:

1.

13. The vector of claim 12 operably linked to control sequences recognized by a host cell transformed with the vector.

14. A host cell transformed with the vector of claim 13.

15. The host cell of claim 14, wherein the host cell comprises a filamentous fungus.

16. The host cell of claim 15, wherein the filamentous fungus is selected from the group consisting of Trichoderma spp., Humicola spp., Neurospora spp., Aspergillus spp., and Fusarium spp.

17. The host cell of claim 16, wherein the filamentous fungus comprises Trichoderma reesei.