Polymer gel contact masks and methods and molds for making same

• US 6,770,721 B1
• Filed: 11/02/2000
• Issued: 08/03/2004
• Est. Priority Date: 11/02/2000
• Status: Expired due to Fees

• Current Assignee: Surface Logix, Inc.
• Original Assignee: Surface Logix, Inc.
• Inventors: Kim, Enoch
• Primary Examiner(s): PENG, KUO LIANG
• Application Number:
  US09/705,187
• Time in Patent Office: 1,370 Days
• Field of Search: 526/328, 526/329, 428/500, 428/523, 428/447, 428/423.1, 435/4
• US Class Current: 526/329
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B29C 2043/023 having a plurality of grooves
• CPC Class Codes: B29C 2043/3652 Elastic moulds or mould parts, e.g. cores or inserts
• CPC Class Codes: B29C 33/405 Elastomers, e.g. rubber B29C33/50 takes precedence
• CPC Class Codes: B29C 35/02 Heating or curing, e.g. crosslinking or vulcanizing during moulding, e.g. in a mouldcold vulcanisation B29C35/18 ; vulcanising tyres, presses therefor B29D30/0601
• CPC Class Codes: B29C 43/021 characterised by the shape of the surface
• CPC Class Codes: B29C 43/10 Isostatic pressing, i.e. using non-rigid pressure-exerting members against rigid parts or dies
• CPC Class Codes: B29C 43/3642 Bags, bleeder sheets or cauls for isostatic pressing
• CPC Class Codes: B29K 2105/0061 Gel or sol
• CPC Class Codes: B29K 2105/256 Sheets, plates, blanks or films
• CPC Class Codes: Y10T 428/31551 Of polyamidoester [polyurethane, polyisocyanate, polycarbamate, etc.]
• CPC Class Codes: Y10T 428/31663 As siloxane, silicone or silane
• CPC Class Codes: Y10T 428/31855 Of addition polymer from unsaturated monomers
• CPC Class Codes: Y10T 428/31938 Polymer of monoethylenically unsaturated hydrocarbon

Abstract

The present invention relates to elastomeric contact masks, and methods of making these contact masks by complementary molding using complementary molds. The contact masks are made of polymer gels, including hydrogel materials. The complementary molds of the present invention include at least one elastomeric half mold. Also disclosed are methods of continuous complementary molding.

Claims

1. A patterning transfer element comprising a polymer gel, where the polymer gel comprises a crosslinked HEMA copolymer;

and where the patterning transfer element has surface features that are elevated portions or recessed portions so as to allow for the patterning of biological materials by the patterning transfer element.

2. The patterning transfer element of claim 1 wherein the patterning transfer element is a stamp.

3. The patterning transfer element of claim 1 wherein the patterning transfer element is applied to an inanimate substrate.

4. The patterning transfer element of claim 1 wherein the patterning transfer element conforms to a surface of a substrate upon contact with the surface.

5. A contact mask comprising a polymer gel and having holes or cavities with a cross-sectional area of from approximately 1 μ

m² to approximately 2 mm².

6. The contact mask of claim 5 wherein the polymer gel is a hydrogel.

7. The contact mask of claim 6 wherein the hydrogel comprises polymer chains of polyHEMA.

8. The contact mask of claim 7 wherein the polymer chains are a homopolymer of HEMA crosslinked with a crosslinking agent.

9. The contact mask of claim 8 wherein the crosslinking agent is a diacrylate or polyacrylate.

10. The contact mask of claim 7 wherein the polymer chains are co-polymers of HEMA, a hydrophobic monomer and, optionally, a crosslinking agent.

11. The contact mask of claim 7 wherein the polymer chains are block co-polymers of HEMA and a biodegradable polymer.

12. The contact mask of claim 6 wherein the hydrogel is formed by polymerization of a polymer precursor composition comprising one or more chemically distinct monomer compounds and a crosslinking agent wherein the crosslinking agent is present in an amount of about 1 mole percent to about 5 mole percent with respect to the total monomer compound content.

13. The contact mask of claim 5 wherein the contact mask ranges from approximately 5 μ

m to approximately 3 mm in thickness.

14. The contact mask of claim 5 wherein the polymer gel is selected from the group consisting of polyphosphazenes;

polyacrylates;

polymethacrylates, poly(ethylene glycol), poly(ethylene glycol) acrylates, poly(vinyl alcohol), polyethylene glycol methacrylates, 2-trimethoxysilyloxy)ethyl methacrylate, trimethoxysilyloxy alkyl methacrylate, trimethoxy silyl alkyl methacrylate, polyvinylpyrrolidinone and carbohydrate-based hydrogel polymers, heparin, heparin sulfate, hyaluronic acid, polylactic acid, polybutadienes, hydrogels, and combinations thereof.

15. The contact mask of claim 5 formed by complementary molding.

16. The contact mask of claim 5 wherein the polymer gel is a hydrogel or polyelectrolyte gel.

17. The contact mask of claim 5 wherein the mask is located on an inanimate substrate.

18. The contact mask of claim 5 wherein the mask is in conformal contact with a surface upon which the mask is located to provide a seal between the mask and the surface.

19. The contact mask of claim 18 wherein the surface is the surface of a culture dish for culturing cells.

20. A hydrogel contact mask where the hydrogel comprises polymer chains of polyHEMA and the polymer chains are co-polymers of HEMA, a hydrophobic monomer and, optionally, a crosslinking agent and where the contact mask has surface features that are elevated portions or recessed portions so as to allow for the patterning of biological materials by the contact mask.

21. A hydrogel contact mask wherein the hydrogel comprises polymer chains of polyHEMA and the polymer chains are block co-polymers of HEMA and a biodegradable polymer and where the contact mask has surface features that are elevated portions or recessed portions so as to allow for the patterning of biological materials by the contact mask.

22. A patterning transfer element comprising a polymer gel where the polymer gel comprises a HEMA copolymer and where the patterning transfer element has surface features that are elevated portions or recessed portions so as to allow for the patterning of biological materials by the patterning transfer element.

23. A contact mask comprising a polymer gel wherein the polymer gel comprises a HEMA copolymer and where the contact mask has surface features that are elevated portions or recessed portions so as to allow for the patterning of biological materials by the contact mask.

24. The contact mask of claim 23 comprising at least one hole therethrough.

25. A contact mask comprising a polymer gel with a surface having a pattern of cavities complementary to an ordered array of posts in a half mold used to produce the contact mask.

26. The contact mask of claim 25 wherein there are cells in the cavities.

27. The contact mask of claim 26 wherein cells are not adhering to the surface of the contact mask.

28. A contact mask having a top and a bottom surface comprising a pattern of cavities in both the top and bottom surfaces where the cavities in the top surface are complementary to an ordered array of posts in a first half mold used to produce the contact mask and where the cavities in the bottom surface are complementary to an ordered array of posts in a second half mold used to produce the contact mask.