Devices for performing array hybridization assays and methods of using the same

• US 6,773,676 B2
• Filed: 06/19/2001
• Issued: 08/10/2004
• Est. Priority Date: 04/27/1999
• Status: Expired due to Fees

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Schembri, Carol T.
• Primary Examiner(s): Ludlow, Jan M.
• Application Number:
  US09/884,792
• Publication Number:
  US 20010046702A1
• Time in Patent Office: 1,148 Days
• Field of Search: 422/102, 422/104, 435/287.2, 435/288.3, 435/305.1
• US Class Current: 422/559
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/041 Connecting closures to device or container
• CPC Class Codes: B01L 2300/0809 rectangular shaped
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: G01N 21/0332 with temperature control control of temperature G05D23/00; cryostats F17C3/08
• CPC Class Codes: G02B 21/34 Microscope slides, e.g. mounting specimens on microscope slides
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

Array hybridization devices and methods for their use are provided. The subject devices are characterized by having a substantially planar bottom surface, a cover, at least one fluid port and at least one adjustable spacing element for adjusting the spacing between an array and the bottom surface. In using the subject devices, an array is placed on the at least one adjustable spacing element in the chamber and the space between the array and the bottom surface is adjusted by moving the at least one adjustable spacing element. The adjusted array is contacted with at least one biological sample introduced into the chamber. The subject inventions find use in a variety of array-based applications, including nucleic acid array hybridizations.

Claims

1. An array hybridization chamber comprising:

a. a bottom surface for receiving at least one array to be hybridized and a cover operatively associated with said bottom surface, wherein said cover forms a sealed enclosure with said bottom surface when in a closed position,

b. at least one adjustable spacing element positioned on said bottom surface for adjusting the spacing between said at least one array and said bottom surface and said cover when said bottom surface and said cover are operatively associated,

c. at least one fluid port for delivering and/or removing fluid from said chamber.

2. The array hybridization chamber according to claim 1, wherein said chamber comprises at least two adjustable spacing elements.

3. The array hybridization chamber according to claim 1, wherein said cover is operatively associated with said substantially planar bottom surface by a hinge.

4. The array hybridization chamber according to claim 1, wherein said cover forms a substantially vapor tight seal with said substantially planar bottom surface when in a closed position.

5. The array hybridization chamber according to claim 1, further comprising at least one mixing element for mixing the contents of said chamber.

6. The array hybridization chamber according to claim 5, wherein said at least one mixing element is selected from the group consisting of at least one resistor, ultrasonic element, recirculation pump, at least one roller, at least one adjustable spacing element and at least one solenoid.

7. The array hybridization chamber according to claim 1, further comprising a temperature regulation system for monitoring and controlling the temperature of said chamber.

8. The array hybridization chamber according to claim 7, wherein said temperature regulation system is selected from the group consisting of a thermo-electric means, a thermo-fluidic means and a heating/cooling block.

9. The array hybridization chamber according to claim 1, further comprising a system for automating at least a portion of said array hybridization chamber.

10. A system for array hybridization, said system comprising:

(a) a hybridization station, and

(b) at least one hybridization chamber according to claim 1.

11. The system according to claim 10, wherein said system further comprises an automation system for automating at least a portion of a hybridization process.

12. The system according to claim 10, further comprising an array.

13. An array hybridization chamber according to claim 1, further comprising an array.

14. An array hybridization chamber comprising:

a. a bottom surface for receiving at least one array to be hybridized and comprising a plurality of micro-channels for introducing a plurality of biological samples into said chamber, such that said plurality of samples remain segregated from each other,

b. at least one adjustable spacing element for adjusting the spacing between said at least one array and said bottom surface,

c. a cover operatively associated with said bottom surface, wherein said cover forms a sealed enclosure with said bottom surface when in a closed position, and

d. at least one fluid port for delivering and/or removing fluid from said chamber.

15. The array hybridization chamber according to claim 14, wherein said micro-channels define discrete locations on said substantially planar bottom surface.

16. The array hybridization chamber according to claim 14, wherein said chamber comprises at least two adjustable spacing elements.

17. The array hybridization chamber according to claim 16, wherein said at least one drying element comprises at least one gas jet.

18. The array hybridization chamber according to claim 14, wherein said cover is operatively associated with said substantially planar bottom surface by a hinge.

19. The array hybridization chamber according to claim 14, wherein said cover forms a substantially vapor tight seal with said substantially planar bottom surface when in a closed position.

20. The array hybridization chamber according to claim 14, further comprising at least one mixing element for mixing the contents of said chamber.

21. The array hybridization chamber according to claim 20, wherein said at least one mixing element is selected from the group consisting of at least one resistor, ultrasonic element, recirculation pump, at least one roller, at least one adjustable spacing element and at least one solenoid.

22. The array hybridization chamber according to claim 14, further comprising a temperature regulation system for monitoring and controlling the temperature of said chamber.

23. The array hybridization chamber according to claim 22, wherein said temperature regulation system is selected from the group consisting of a thermo-electric means, a thermo-fluidic means and a heating/cooling block.

24. An array hybridization chamber comprising:

a. a bottom surface for receiving at least one array to be hybridized,

b. at least one adjustable spacing element for adjusting the spacing between said at least one array and said bottom surface,

c. a cover operatively associated with said bottom surface, wherein said cover forms a sealed enclosure with said bottom surface when in a closed position,

d. at least one fluid port for delivering and/or removing fluid from said chamber, and

e. at least one drying element for drying said at least one array.

25. The array hybridization chamber according to claim 24, wherein said chamber comprises at least two adjustable spacing elements.

26. The array hybridization chamber according to claim 24, wherein said cover is operatively associated with said substantially planar bottom surface by a hinge.

27. The array hybridization chamber according to claim 24, wherein said cover forms a substantially vapor tight seal with said substantially planar bottom surface when in a closed position.

28. The array hybridization chamber according to claim 24, further comprising at least one mixing element for mixing the contents of said chamber.

29. The array hybridization chamber according to claim 28, wherein said at least one mixing element is selected from the group consisting of at least one resistor, ultrasonic element, recirculation pump, at least one roller, at least one adjustable spacing element and at least one solenoid.

30. The array hybridization chamber according to claim 24, further comprising a temperature regulation system for monitoring and controlling the temperature of said chamber.

31. The array hybridization chamber according to claim 30, wherein said temperature regulation system is selected from the group consisting of a thermo-electric means, a thermo-fluidic means and a heating/cooling block.