Devices for performing array hybridization assays and methods of using the same
First Claim
1. An array hybridization chamber comprising:
- a. a bottom surface for receiving at least one array to be hybridized and a cover operatively associated with said bottom surface, wherein said cover forms a sealed enclosure with said bottom surface when in a closed position, b. at least one adjustable spacing element positioned on said bottom surface for adjusting the spacing between said at least one array and said bottom surface and said cover when said bottom surface and said cover are operatively associated, c. at least one fluid port for delivering and/or removing fluid from said chamber.
1 Assignment
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Accused Products
Abstract
Array hybridization devices and methods for their use are provided. The subject devices are characterized by having a substantially planar bottom surface, a cover, at least one fluid port and at least one adjustable spacing element for adjusting the spacing between an array and the bottom surface. In using the subject devices, an array is placed on the at least one adjustable spacing element in the chamber and the space between the array and the bottom surface is adjusted by moving the at least one adjustable spacing element. The adjusted array is contacted with at least one biological sample introduced into the chamber. The subject inventions find use in a variety of array-based applications, including nucleic acid array hybridizations.
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Citations
31 Claims
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1. An array hybridization chamber comprising:
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a. a bottom surface for receiving at least one array to be hybridized and a cover operatively associated with said bottom surface, wherein said cover forms a sealed enclosure with said bottom surface when in a closed position, b. at least one adjustable spacing element positioned on said bottom surface for adjusting the spacing between said at least one array and said bottom surface and said cover when said bottom surface and said cover are operatively associated, c. at least one fluid port for delivering and/or removing fluid from said chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
(a) a hybridization station, and (b) at least one hybridization chamber according to claim 1.
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11. The system according to claim 10, wherein said system further comprises an automation system for automating at least a portion of a hybridization process.
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12. The system according to claim 10, further comprising an array.
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13. An array hybridization chamber according to claim 1, further comprising an array.
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14. An array hybridization chamber comprising:
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a. a bottom surface for receiving at least one array to be hybridized and comprising a plurality of micro-channels for introducing a plurality of biological samples into said chamber, such that said plurality of samples remain segregated from each other, b. at least one adjustable spacing element for adjusting the spacing between said at least one array and said bottom surface, c. a cover operatively associated with said bottom surface, wherein said cover forms a sealed enclosure with said bottom surface when in a closed position, and d. at least one fluid port for delivering and/or removing fluid from said chamber. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23)
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24. An array hybridization chamber comprising:
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a. a bottom surface for receiving at least one array to be hybridized, b. at least one adjustable spacing element for adjusting the spacing between said at least one array and said bottom surface, c. a cover operatively associated with said bottom surface, wherein said cover forms a sealed enclosure with said bottom surface when in a closed position, d. at least one fluid port for delivering and/or removing fluid from said chamber, and e. at least one drying element for drying said at least one array. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31)
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Specification