Apoptosis-inducing factor

• US 6,773,911 B1
• Filed: 11/23/1999
• Issued: 08/10/2004
• Est. Priority Date: 11/23/1998
• Status: Expired due to Term

• Current Assignee: Amgen Canada Incorporated (Amgen Inc.)
• Original Assignee: Amgen Canada Incorporated (Amgen Inc.), Centre National De La Recherche Scientifique
• Inventors: Zamzami, Naoufal, Siderovski, David Peter, Snow, Bryan E. L., Penninger, Josef M., Susin, Santos A., Kroemer, Guido P.
• Primary Examiner(s): Canella, Karen A.
• Application Number:
  US09/447,497
• Time in Patent Office: 1,722 Days
• Field of Search: 536/23.1, 536/23.5, 536/24.31, 530/350, 514/2, 435/320.1, 435/252.3, 435/254.11, 435/252.33, 435/252.34, 435/252.35, 435/254.21, 435/254.22, 435/254.23, 435/255.6, 435/255.4
• US Class Current: 435/243
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/4747 Apoptosis related proteins

Abstract

The present invention related generally novel mammalian apoptosis-inducing factors, polynucleotides encoding such factors and methods related thereto.

Claims

1. An isolated polynucleotide comprising the nucleotide sequence of SEQ ID NO:

1.

16. An isolated cloning vector which comprises a polynucleotide according to any one of claims 1-3, 4-6, 7-9, 10-12, and 13-15.

17. An isolated expression vector which comprises a polynucleotide according to any one of claims 1-3, 4-6, 7-9, 10-12, and 13-15.

18. An isolated expression vector which comprises a polynucleotide according to any one of claims 1-3, 4-6, 7-9, 10-12, and 13-15, operatively associated with an expression control sequence.

19. The isolated expression vector of claim 18 wherein said expression control sequence is selected from the group consisting of the immediate early prometers of human cytomegalovirus (hCMV), early prometers of SV-40, early promoters of adenovirus, early promoters of polyoma virus, late promoters of SV-40, late promoters of vaccinia virus, late promoters of polyoma virus, retroviral LTR, inducible promoters, promoters of the lac system, promoters of the trp system, promoters TAC system, promoters of the TRC system, the major operators and promoter regions of phage lambda, control regions of fd coat protein, 3-phosphoglycerate kinase promoter, acid phosphatase promoter, and promoters of yeast α

mating factor.

20. An isolated unicellular host transformed with a polynucleotide according to any one of claims 1-3, 4-6, 7-9, 10-12, and 13-15.

21. The isolated unicellular host of claim 20 wherein the isolated host cell is selected from the group consisting of E. coli, Pseudomonas, Bacillus, Streptomyces, yeast, CHO, R1.1, B-W, LM, C051, C057, BSC1, BSC40, BMT10 and SF9 cells.

22. The isolated unicellular host according to claim 21 wherein the isolated unicellular host is a yeast selected from the group consisting of Saccharomyces, Pichia, Candida, Hansenula, and Toulopsis.

23. A method for producing an apoptosis-inducing factor polypeptide, the method comprising the steps of:

(a) culturing the host cell of claim 20 under conditions suitable for the expression of the apoptosis-inducing factor polypeptide; and

(b) recovering the expressed apoptosis-including factor polypeptide.

2. An isolated polynucleotide comprising a sequence that encodes a polypeptide having the amino acid sequence of SEQ ID NO:

2.

3. An isolated polynucleotide comprising a sequence that encodes a polypeptide having the amino acid sequence of SEQ ID NO:

3.

4. An isolated polynucleotide comprising the nucleotide sequence of SEQ ID NO:

4.

5. An isolated polynucleotide comprising a sequence that encodes a polypeptide having the amino acid sequence of SEQ ID NO:

5.

6. An isolated polynucleotide comprising a sequence that encodes a polypeptide having the amino acid sequence of SEQ ID NO:

6.

7. An isolated polynucleotide comprising the nucleotide sequence of SEQ ID NO:

7.

8. An isolated polynucleotide comprising a sequence that encodes a polypeptide having the amino acid sequence of SEQ ID NO:

8.

9. An isolated polynucleotide comprising a sequence that encodes a polypeptide having the amino acid sequence of SEQ ID NO:

9.

10. An isolated polynucleotide comprising the nucleotide sequence of SEQ ID NO:

10.

11. An isolated polynucleotide comprising a sequence that encodes a polypeptide having the amino acid sequence of SEQ ID NO:

11.

12. An isolated polynucleotide comprising a sequence that encodes a polypeptide having the amino acid sequence of SEQ ID NO:

12.

13. An isolated polynucleotide comprising the nucleotide sequence of SEQ ID NO:

13.

14. An isolated polynucleotide comprising a sequence that encodes a polypeptide having the amino acid sequence of SEQ ID NO:

14.

15. An isolated polynucleotide comprising a sequence that encodes a polypeptide having the amino acid sequence of SEQ ID NO:

15.

24. A diagnostic reagent comprising a detectably labeled polynucleotide encoding human apoptosis inducing factor comprising an amino acid sequence selected from the group consisting of SEQ ID NO:

8, SEQ ID NO;

9, SEQ ID NO;

11, SEQ ID NO;

12, SEQ ID NO;

14, and SEQ ID NO;

15.

27. A diagnostic reagent of claim 24 or claim 25 wherein said labeled polynucleotide is a first-strand cDNA.

25. A diagnostic reagent comprising a detectably labeled polynucleotide encoding murine apoptosis inducing factor comprising an amino acid sequence selected from the group consisting of SEQ ID NO:

2, SEQ ID NO;

3, SEQ ID NO;

5, and SEQ ID NO;

6.

26. The diagnostic reagent of claim 4 or claim 25 wherein said labeled polynucleotide is a DNA.