Biosensor

• US 6,776,888 B2
• Filed: 03/26/2002
• Issued: 08/17/2004
• Est. Priority Date: 07/31/2000
• Status: Expired due to Fees

• Current Assignee: Panasonic Healthcare Holdings Co., Ltd.
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Yamamoto, Tomohiro, Ikeda, Shin, Nankai, Shiro, Watanabe, Motokazu, Hasegawa, Miwa
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US10/089,289
• Publication Number:
  US 20020148726A1
• Time in Patent Office: 875 Days
• Field of Search: 204/403.01, 204/403.05, 204/403.06, 204/403.09, 204/403.1, 204/403.14
• US Class Current: 204/403.09
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

The present invention provides a biosensor that enables highly-accurate measurement of a sample solution including a solid component like hemocytes and has a little variation in response. The biosensor includes: an insulating base plate, an electrode system having at least a working electrode and a counter electrode provided on the base plate, a cover member that is combined with the base plate to define a sample solution supply pathway for leading a sample solution from a sample supply unit to the electrode system, a reaction reagent system including at least an oxidation-reduction enzyme and an electron mediator, and a filter disposed between the electrode system and the sample supply unit in the sample solution supply pathway. The biosensor has a space that encircles surface of the filter in an area from one end of the filter close to the sample supply unit to the other end of the filter close to the electrode system. This arrangement effectively prevents the solid component like hemocytes from flowing into the electrode system without being filtered out by the filter.

Claims

1. A biosensor comprising:

an insulating base plate,

an electrode system that is provided on the base plate and has at least a working electrode and a counter electrode,

a cover member that is combined with the base plate to define a sample solution supply pathway for leading a sample solution from a sample supply unit to the electrode system, wherein the sample supply unit is located on a side of the electrode system,

a reaction reagent system including at least an oxidation-reduction enzyme and an electron mediator, and

a filter in the sample solution supply pathway, the filter disposed between the electrode system and the sample supply unit,

the biosensor having a space that encircles the surface of the filter in an area located between a first end of the filter close to the sample supply unit and a second end of the filter close to the electrode system, wherein said space has a width of 0.5 mm to 5.0 mm.

2. The biosensor of claim 1, wherein the cover member is disposed above the base plate, and the sample solution supply pathway starts from the sample supply unit provided on the base plate and is formed along the cover member and the base plate.

3. The biosensor of claim 1, wherein the space has a width of 1.0 mm to 3.0 mm.

4. The biosensor of claim 1, wherein the filter is a porous body having spaces connecting with one another in a three-dimensional manner, and the porous body moves blood from the sample supply unit toward the sample solution supply pathway by capillarity and functions to filter hemocytes based on a difference between the flow resistance of plasma and the flow resistance of hemocytes.

5. The bio sensor of claim 1, wherein the oxidation-reduction enzyme is cholesterol oxidase.

6. The biosensor of claim 1, wherein the reaction reagent system includes an enzyme having an ability of hydrolyzing cholesterol ester.

7. The biosensor of claim 6, wherein the enzyme having the ability of hydrolyzing cholesterol ester is cholesterol esterase.

8. The biosensor of claim 1, wherein the reaction reagent system includes a surface active agent.

9. The biosensor of claim 1, wherein part or all of the cover member and of the insulating base plate are transparent.

10. A biosensor comprising:

an insulating base plate,

an electrode system that is provided on the base plate and has at least a working electrode and a counter electrode,

a cover member that is combined with the base plate to define a sample solution supply pathway for leading a sample solution from a sample supply unit to the electrode system, wherein the sample solution supply pathway is disposed in a direction of gravity from the sample supply unit provided on the cover member,

a reaction reagent system including at least an oxidation-reduction enzyme and an electron mediator, and

a filter in the sample solution supply pathway, the filter disposed between the electrode system and the sample supply unit,

the biosensor having a space that encircles the surface of the filter in an area located between a first end of the filter close to the sample supply unit and a second end of the filter close to the electrode system, wherein the width of the space is not less that 100 μ

m and is smaller than the thickness of the filter.

11. The biosensor of claim 10, wherein the sample supply unit is located above the electrode system.

12. The biosensor of claim 10, wherein the filter is a porous body having spaces connecting with one another in a three-dimensional manner, and the porous body moves blood from the sample supply unit toward the sample solution supply pathway by capillarity and functions to filter hemocytes based on a difference between the flow resistance of plasma and the flow resistance of hemocytes.

13. The biosensor of claim 10, wherein the oxidation-reduction enzyme is cholesterol oxidase.

14. The biosensor of claim 10, wherein the reaction reagent system includes an enzyme having an ability of hydrolyzing cholesterol ester.

15. The biosensor of claim 14, wherein the enzyme having the ability of hydrolyzing cholesterol ester is cholesterol esterase.

16. The biosensor of claim 10, wherein the reaction reagent system includes a surface active agent.

17. The biosensor of claim 10, wherein part or all of the cover member and of the insulating base plate are transparent.