Particulate material for purification of bio-macromolecules
First Claim
1. A particulate material having a density of at least 2.5 g/ml, comprised of particles having an average diameter of 5-75 μ
- m, wherein said particles of the particulate material are essentially constructed of a polymeric base matrix and a non-porous core material, said core material having a density of at least 3.0 g/ml, and said polymeric base matrix comprising either chargeable pendant groups or affinity ligands for a bio-molecule.
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Abstract
The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 μm, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.
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Citations
32 Claims
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1. A particulate material having a density of at least 2.5 g/ml, comprised of particles having an average diameter of 5-75 μ
- m, wherein said particles of the particulate material are essentially constructed of a polymeric base matrix and a non-porous core material, said core material having a density of at least 3.0 g/ml, and said polymeric base matrix comprising either chargeable pendant groups or affinity ligands for a bio-molecule.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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32. A particulate material having a density of in the range of 3.2-5.0 g/ml, comprised of particles having an average diameter of 15-45 μ
- m, and the particles of the particulate material are essentially constructed of a polysaccharide base matrix and a core material, said core material having a density in the range of 6.0-12.0 g/m3, said polysaccharide base matrix including pendant groups selected from polyethyleneimine chains, modified polyethyleneimine chains and poly(ethyleneimine/oxyethylene) chains, said pendant groups forming a tentacular structure on the surface of the particle.
Specification