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Methods for quantitative and qualitative analyses of phospholipids using one-dimensional thin layer chromatography

  • US 6,783,988 B1
  • Filed: 10/19/2000
  • Issued: 08/31/2004
  • Est. Priority Date: 10/19/2000
  • Status: Expired due to Term
First Claim
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1. A method for the simultaneous quantitative and qualitative determination of individual phospholipids in a phospholipid mixture, wherein said phospholipid mixture comprises a neutral lipid, said method comprising:

  • dissolving said phospholipid mixture in at least one extraction solvent;

    applying said at least one extraction solvent having said phospholipid mixture dissolved therein to a thin layer chromatography (TLC) plate;

    placing said TLC plate having said phospholipid mixture applied thereto into an elution solvent mixture comprising chloroform, methanol, acetic acid, and an aqueous solution of potassium chloride; and

    allowing said phospholipid mixture to migrate up said TLC plate in one direction until said individual phospholipids are resolved into discrete, detectable spots such that all said individual phospholipids present in said phospholipid mixture can be individually detected and said discrete detectable spots are separately quantifiable.

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