Target molecules detection by waveguiding in a photonic silicon membrane

• US 6,785,432 B2
• Filed: 05/31/2002
• Issued: 08/31/2004
• Est. Priority Date: 06/15/2001
• Status: Expired due to Fees

• Current Assignee: Lawrence Livermore National Security LLC (Government of the United States of America)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Letant, Sonia, Terminello, Louis, Van Buuren, Anthony
• Primary Examiner(s): Healy, Brian
• Assistant Examiner(s): Wood, Kevin S.
• Application Number:
  US10/159,175
• Publication Number:
  US 20020191884A1
• Time in Patent Office: 823 Days
• Field of Search: 385/12, 385/14, 385/129-132, 250/227.11, 250/227.14, 422/82-5-
• US Class Current: 385/12
• CPC Class Codes: G01N 2021/7783 Transmission, loss
• CPC Class Codes: G01N 21/774 the reagent being on a grating or periodic structure
• CPC Class Codes: G01N 21/783 for analysing gases
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/553 Metal or metal coated

Abstract

Disclosed herein is a photonic silicon filter capable of binding and detecting biological and chemical target molecules in liquid or gas samples. A photonic waveguiding silicon filter with chemical and/or biological anchors covalently attached to the pore walls selectively bind target molecules. The system uses transmission curve engineering principles to allow measurements to be made in situ and in real time to detect the presence of various target molecules and determine the concentration of bound target.

Claims

1. A photonic waveguiding device comprising:

at least one silicon wafer having a plurality of through pores distributed according to a designed pattern leading to a photonic band gap; and

at least one chemical or biological target specific anchor attached to the inner wall of at least one of said pores, wherein said anchor is capable of binding to a specific chemical or biological target molecule.

2. The photonic waveguiding device recited in claim 1, wherein said pores have pore diameters ranging in size from about 50 nm to about 1 μ

m.

3. The photonic waveguiding device recited in claim 1, wherein said anchor is attached to said pore wall via silicon-carbon or silicon-oxygen-carbon covalent bonding.

4. The photonic waveguiding device recited in claim 1, wherein the total size of said device has dimensions no larger than 200 μ

m×

1 cm×

1 cm.

5. A photonic waveguiding device comprising:

an array of waveguiding filters, wherein each filter is functionalized with a chemical or biological target specific anchor to allow the contemporaneous detection of various chemical and biological target molecules and wherein each of said filters comprise (1) a silicon wafer having a plurality of through pores distributed according to a designed pattern leading to a photonic band gap and (2) a chemical or biological target specific anchor attached to the inner wall of at least one of said pores, said anchor being capable of binding to a chemical or biological target molecule.

6. A photonic waveguiding detection system comprising:

a light source;

at least one silicon waveguiding filter, wherein said filter comprises a silicon wafer having (1) a plurality of through pores distributed according to a designed pattern leading to a photonic band gap and (2) at least one chemical or biological target specific anchor attached to the inner wall of at least one of said pores, wherein said anchor is capable of binding to a chemical or biological target molecule;

a detector to count the photons transmitted through the device; and

a computer to analyze the light transmitted through the filter by (1) recording the intensity and wavelength of light transmitted through the filter, (2) identifying the presence of target molecules bound in the device and (3) determining the concentration of bound target molecules.

7. A method comprising:

measuring the transmission curve through at least one silicon filtering device, wherein said filtering device comprises (1) a plurality of through pores distributed according to a designed pattern leading to a photonic band gap and (2) at least one chemical or biological target specific anchor attached to the inner wall of at least one of said pores, wherein said anchor is capable of binding to a chemical or biological target molecule;

passing a sample through said silicon filter, said sample being a gas or a liquid;

shining a light orthogonal to the pores of said silicon filter, while contemporaneously flowing said sample through said filter; and

measuring the transmission curve of said waveguiding silicon filter as said sample passes through said filter, wherein modifications in said transmission curve are (1) indicative that at least one of said target molecules has bound to said anchor and (2) indicative of the concentration of said bound target molecules.

8. The method of claim 7, further comprising:

releasing the bound target molecules from the pore walls after measuring the transmission curve, wherein said transmission curve was generated by passing said sample through said filtering device.

9. The method of claim 8, further comprising:

regenerating said filtering device after releasing the bound target molecules from the pore walls by adding a plurality of chemical or biological target specific anchor molecules to the pores of said filtering device, said anchor molecules being capable of binding to specific chemical or biological target molecules.

10. A method comprising:

fabricating a silicon membrane with an array of pores designed for opening a photonic band gap and for waveguiding;

functionalizing the pore walls of the silicon membrane with chemical functional groups; and

attaching biological or chemical anchors to the functionalized pore walls of said membrane to create a selective silicon photonic waveguiding filter, wherein the anchors contain chemically or biologically active receptor groups at the end not attached to the pore walls.