Methods for conducting electrophoretic analysis
First Claim
1. A method for separating a mixture of ions in a sample employing a microfluidic device comprising a microchannel having a neutral substantially saturated norbornene based polymer surface and two electrodes for creating an electrical field in said microchannel, said method comprising:
- introducing said sample into said microchannel comprising an aqueous dispersion of a sieving polymer under the electrical influence of said field, whereby ions in said sample migrate in said aqueous dispersion to separate into fractions.
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Abstract
Capillary electrophoresis is performed under conventional conditions in microchannels having a norbornene based polymer surface. The norbornene based polymers can be used as a solid substrate for forming the necessary features for a microfluidic device, where the entire device may be made of norbornene based polymer. Conveniently, a norbornene based polymer layer having a lower glass transition temperature may be used to adhere a cover or enclosing layer to the substrate to enclose the microchannels and provide a bottom for the reservoirs.
105 Citations
5 Claims
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1. A method for separating a mixture of ions in a sample employing a microfluidic device comprising a microchannel having a neutral substantially saturated norbornene based polymer surface and two electrodes for creating an electrical field in said microchannel, said method comprising:
introducing said sample into said microchannel comprising an aqueous dispersion of a sieving polymer under the electrical influence of said field, whereby ions in said sample migrate in said aqueous dispersion to separate into fractions. - View Dependent Claims (2, 3, 4, 5)
Specification