Dynamic coating with linear polymer mixture for electrophoresis
First Claim
1. An electrophoresis channel sieving and coating composition, for use in a microfluidics device, comprising:
- a buffered aqueous solution of from about 0.05 to 7.5 wt % each of a sieving polymer and a channel coating polymer in a weight ratio of 1-10;
1, wherein said coating polymer is less water soluble than said sieving polymer.
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Accused Products
Abstract
Compositions and methods are provided for performing capillary electrophoresis using a composition comprising in combination in an aqueous buffered medium a coating polymer and a sieving polymer, where the sieving polymer is more hydrophilic than the coating polymer and is present in greater amount. Of particular interest are uncrosslinked acrylamide polymer mixtures for coating plastic channels and providing sieving for performing DNA separations in microfluidic devices. Polyacrylamide or N,N-dimethyl acrylamide is used with a N,N-dialkyl acrylamide copolymer, either separately or together for sieving and coating, serving as the medium in capillary electrophoresis DNA separations.
170 Citations
15 Claims
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1. An electrophoresis channel sieving and coating composition, for use in a microfluidics device, comprising:
- a buffered aqueous solution of from about 0.05 to 7.5 wt % each of a sieving polymer and a channel coating polymer in a weight ratio of 1-10;
1, wherein said coating polymer is less water soluble than said sieving polymer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
- a buffered aqueous solution of from about 0.05 to 7.5 wt % each of a sieving polymer and a channel coating polymer in a weight ratio of 1-10;
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11. A microfluidics electrophoresis device comprising a polymer substrate having a separation channel formed therein, contained within said channel, a composition comprising a buffered aqueous solution of from about 0.05 to 7.5% by weight each of a sieving polymer and a channel coating polymer in a weight ratio of 1-10:
- 1, wherein said coating polymer is less water soluble than said sieving polymer.
- View Dependent Claims (12, 13, 14, 15)
Specification