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Arrayed biomolecules and their use in sequencing

  • US 6,787,308 B2
  • Filed: 01/30/2001
  • Issued: 09/07/2004
  • Est. Priority Date: 07/30/1998
  • Status: Expired due to Term
First Claim
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1. An array of nucleic acids comprising;

  • a) a support having a solid surface;

    b) a plurality of distinct optically resolvable polynucleotide molecules immobilized at discrete sites on said solid surface;

    c) an attachment between said solid surface and each of said polynucleotide molecules for the immobilization of said polynucleotide molecules on said solid surface;

    d) wherein each of said polynucleotide molecules comprises a polynucleotide duplex covalently linked to form a hairpin loop structure;

    e) wherein one end of said polynucleotide duplex comprises a target polynucleotide and each polynucleotide duplex is an individually resolvable molecule detectable as a single molecule fluorescent point; and

    f) wherein fluorescence from said single molecule fluorescent point exhibits single step photobleaching and said attachment does not exhibit fluorescence.

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