Arrayed biomolecules and their use in sequencing
First Claim
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1. An array of nucleic acids comprising;
- a) a support having a solid surface;
b) a plurality of distinct optically resolvable polynucleotide molecules immobilized at discrete sites on said solid surface;
c) an attachment between said solid surface and each of said polynucleotide molecules for the immobilization of said polynucleotide molecules on said solid surface;
d) wherein each of said polynucleotide molecules comprises a polynucleotide duplex covalently linked to form a hairpin loop structure;
e) wherein one end of said polynucleotide duplex comprises a target polynucleotide and each polynucleotide duplex is an individually resolvable molecule detectable as a single molecule fluorescent point; and
f) wherein fluorescence from said single molecule fluorescent point exhibits single step photobleaching and said attachment does not exhibit fluorescence.
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Abstract
A device comprising an array of molecules immobilised on a solid surface is disclosed, wherein the array has a surface density which allows each molecule to be individually resolved, e.g. by optical microscopy. Therefore, the arrays of the present invention consist of single molecules are more spatially distinct than the arrays of the prior art.
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11 Claims
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1. An array of nucleic acids comprising;
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a) a support having a solid surface;
b) a plurality of distinct optically resolvable polynucleotide molecules immobilized at discrete sites on said solid surface;
c) an attachment between said solid surface and each of said polynucleotide molecules for the immobilization of said polynucleotide molecules on said solid surface;
d) wherein each of said polynucleotide molecules comprises a polynucleotide duplex covalently linked to form a hairpin loop structure;
e) wherein one end of said polynucleotide duplex comprises a target polynucleotide and each polynucleotide duplex is an individually resolvable molecule detectable as a single molecule fluorescent point; and
f) wherein fluorescence from said single molecule fluorescent point exhibits single step photobleaching and said attachment does not exhibit fluorescence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification