Chemical imaging fiberscope

• US 6,788,860 B1
• Filed: 07/19/2000
• Issued: 09/07/2004
• Est. Priority Date: 07/19/1999
• Status: Expired due to Term

• Current Assignee: Chemimage Corporation
• Original Assignee: Chemimage Corporation
• Inventors: Keitzer, Scott A., Smith, Ryan D., Nelson, Matthew P., Treado, Patrick J.
• Primary Examiner(s): Robinson, Mark A.
• Assistant Examiner(s): AMARI, ALESSANDRO V
• Application Number:
  US09/619,371
• Time in Patent Office: 1,511 Days
• Field of Search: 385/115, 385/116, 385/117, 385/119, 356/301
• US Class Current: 385/117
• CPC Class Codes: A61B 1/00096 Optical elements
• CPC Class Codes: A61B 1/042 characterised by a proximal camera, e.g. a CCD camera
• CPC Class Codes: A61B 5/0075 by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy A61B5/0071 takes precedence
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence
• CPC Class Codes: G01N 2021/656 Raman microprobe
• CPC Class Codes: G01N 21/65 Raman scattering
• CPC Class Codes: G02B 23/26 using light guides for illumination G02B23/2469
• CPC Class Codes: G02B 6/32 having lens focusing means positioned between opposed fibre ends with lens being an integral part of the single fibre end G02B6/262

Abstract

A fiberscope device is disclosed which is suitable for video imaging, laser Raman spectroscopy and laser Raman spectroscopic (i.e. chemical) imaging. The fiberscope design minimizes fiber background interference arising from the laser delivery fiber optic and the coherent fiber optic light gathering bundle while maintaining high light throughput efficiency through the use of integrated spectral filters. In the fiberscope design, the laser delivery fiber optic is offset from the coherent fiber optic light gathering bundle. The laser delivery field is captured entirely by the light gathering field of view of the coherent fiber bundle. The fiberscope incorporates spectral filter optical elements that provide environmental insensitivity, particularly to temperature and moisture. The fiberscope is suited to the analysis of a wide range of condensed phase materials (solids and liquids), including the analysis of biological materials such as breast tissue lesions and arterial plaques, in such a manner to delineate abnormal from normal tissues.

Claims

1. A Raman imaging fiberscope for the collection of white light images, Raman chemical images and Raman spectra from a sample comprising:

an outer jacket;

one or more white light illumination fibers, disposed in said outer jacket, for transmitting white light from a white light source to said sample;

one or more laser illumination fibers, disposed in said outer jacket, for transmitting laser light of a specific laser excitation wavelength from a laser source to said sample;

a coherent fiber bundle, disposed in said outer jacket, for transmitting a white light image of said sample and a Raman chemical image of said sample based on light scattered, reflected or emitted from said sample from one end of said fiber bundle proximate said sample to the opposite end of said fiber bundle distal said sample;

a laser bandpass filter positioned between said one or more laser illumination fibers and said sample for transmitting said laser light of said specific laser excitation wavelength and rejecting light of other wavelengths;

a laser rejection filter positioned between said sample and said coherent fiber bundle for transmitting wavelengths of light other than said specific laser excitation wavelength;

wherein said white light images, said Raman chemical images and said Raman spectra are all collected through said coherent fiber bundle; and

a spatial filter positioned between said sample and said coherent fiber bundle for restricting the field of view of said coherent fiber bundle.

2. The Raman imaging fiberscope of claim 1 wherein said laser bandpass and said laser rejection filters exhibit a temperature dependant bandshift coefficient of about 0.005 nm per degree centigrade or less.

3. The Raman imaging fiberscope of claim 1 further comprising one or more lenses positioned between said sample and said coherent fiber bundle.

4. The Raman imaging fiberscope of claim 1 wherein said laser bandpass and said laser rejection filters are metal oxide dielectric filters.

5. The Raman imaging fiberscope of claim 1 further comprising an optically transparent window disposed at the end of said outer jacket proximate said sample.

6. The Raman imaging fiberscope of claim 5 wherein said window is composed of a material selected from a group comprising quartz, diamond and sapphire.

7. The fiberscope of claim 1 wherein said laser bandpass filter is spatially patterned into a first portion for filtering said laser light and a second, transparent portion.

8. The Raman imaging fiberscope of claim 1 further comprising:

a liquid crystal tunable filter imaging spectrometer coupled to the distal end of said coherent fiber bundle;

wherein said Raman chemical images are collected by tuning said liquid crystal tunable filter over a range of wavelengths and collecting images for each of said tuned wavelengths over a plurality of spatial locations on the surface of said sample, said spatial locations corresponding to individual fibers in said coherent fiber bundle.

9. The Raman imaging fiberscope of claim 8 further comprising a CCD camera, coupled to the output of said liquid crystal tunable filter imaging spectrometer, for viewing said Raman chemical images.

10. The Raman imaging fiberscope of claim 8 further comprising:

a video CCD; and

a video monitor for the viewing of white light images.

11. A Raman imaging fiberscope for the collection of white light images, Raman chemical images and Raman spectra from a sample comprising:

an outer jacket;

one or more white light illumination fibers, disposed in said outer jacket, for transmitting white light from a white light source to said sample;

one or more laser illumination fibers, disposed in said outer jacket, for transmitting laser light of a specific laser excitation wavelength from a laser source to said sample;

a coherent fiber bundle, disposed in said outer jacket, for transmitting a white light image of said sample and scattered Raman light from said sample;

a laser bandpass filter positioned between said one or more laser illumination fibers and said sample for transmitting said laser light of a specific laser excitation wavelength and rejecting light of other wavelengths;

a laser rejection filter positioned between said sample and said coherent fiber bundle for transmitting wavelengths of light other than said specific laser excitation wavelength; and

a liquid crystal tunable filter imaging spectrometer; and

a spatial filter positioned between said sample and said coherent fiber bundle for restricting the field of view of said coherent fiber bundle.

12. The Raman imaging fiberscope of claim 11 further comprising:

a mount for holding said fiberscope in proximity to said sample;

a link for directing the output of said fiberscope under white light illumination conditions to a video CCD for viewing on a video monitor;

a link for directing the output of said fiberscope under laser illumination conditions to a Raman spectrometer; and

a link for directing the output of said fiberscope under laser illumination conditions to said liquid crystal tunable filter imaging spectrometer.

13. The Raman imaging fiberscope of claim 12 further comprising software and hardware for producing and displaying a Raman chemical image of said sample.

14. A Raman imaging fiberscope for collecting white light images, Raman chemical images and Raman spectra from a sample comprising:

one or more white light illumination fibers for transmitting white light from a white light source to said sample;

one or more laser illumination fibers for transmitting laser light of a specific laser excitation wavelength from a laser source to said sample;

a coherent fiber bundle;

a laser bandpass filter positioned between said one or more laser illumination fibers and said sample for transmitting said laser light of a specific laser excitation wavelength and rejecting light of other wavelengths;

a laser rejection filter positioned between said sample and said coherent fiber bundle for transmitting wavelengths of light other than said specific laser excitation wavelength; and

a spatial filter positioned between said sample and said coherent fiber bundle for restricting the field of view of said coherent fiber bundle;

wherein said coherent fiber bundle transmits white light images, images composed of Raman scattered light and Raman spectra from a plurality of locations on the surface of said sample corresponding to individual fibers in said coherent fiber bundle.

15. The Raman imaging fiberscope of claim 14 wherein said laser bandpass and said laser rejection filters exhibit a temperature dependant bandshift coefficient of about 0.005 nm per degree centigrade or less.

16. The Raman imaging fiberscope of claim 14 further comprising one or more lenses positioned between said sample and said coherent fiber bundle.

17. The Raman imaging fiberscope of claim 14 further comprising an outer jacket for enclosing said fiberscope, said outer jacket containing said white light illumination fibers, said laser illumination fibers and said coherent fiber bundle.

18. The Raman imaging fiberscope of claim 17 further comprising an optically transparent window disposed at the end of said outer jacket.

19. The Raman imaging fiberscope of claim 18 wherein said window is composed of a material selected from a group comprising quartz, diamond and sapphire.

20. The Raman imaging fiberscope of claim 14 wherein said laser bandpass filter is spatially patterned into a first portion for filtering said laser light and a second, transparent portion.

21. The Raman imaging fiberscope of claim 14 wherein said laser bandpass and said laser rejection filters are metal oxide dielectric filters.

22. The Raman imaging fiberscope of claim 14 further comprising:

a mount for holding said fiberscope in proximity to said sample;

a link for directing the output of said fiberscope under white light illumination conditions to a video CCD for viewing on a video monitor;

a link for directing the output of said fiberscope under laser illumination conditions to a Raman spectrometer; and

a link for directing the output of said fiberscope under laser illumination conditions to said liquid crystal tunable filer imaging spectrometer.

23. The Raman imaging fiberscope of claim 22 further comprising software and hardware for producing and displaying a Raman chemical image of said sample.

24. A Raman imaging fiberscope for collecting broadband images, Raman chemical images and Raman spectra from a sample comprising:

one or more white light illumination fibers for transmitting white light from a white light source to said sample;

one or more laser illumination fibers for transmitting laser light of a specific laser excitation wavelength from a laser source to said sample;

a coherent fiber bundle;

a laser bandpass filter positioned between said one or more laser illumination fibers and said sample for transmitting said laser light of a specific laser excitation wavelength and rejecting light of other wavelengths;

a laser rejection filter positioned between said sample and said coherent fiber bundle for transmitting wavelengths of light other than said specific laser excitation wavelength;

one of more lenses positioned between said sample and said coherent fiber bundle;

a spatial filter position between said sample and said coherent fiber bundle for restricting the field of view of said coherent fiber bundle;

an outer jacket for angular enclosing the fiberscope; and

a window disposed at the end of said outer jacket.