Dot printer with off-axis loading

• US 6,789,965 B2
• Filed: 05/31/2002
• Issued: 09/14/2004
• Est. Priority Date: 05/31/2002
• Status: Expired due to Fees

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Barth, Phillip W, Gordon, Gary B.
• Primary Examiner(s): Hirshfeld, Andrew H.
• Assistant Examiner(s): Ghatt, Dave A.
• Application Number:
  US10/159,415
• Publication Number:
  US 20030223796A1
• Time in Patent Office: 837 Days
• Field of Search: 400/124.28, 400/124.29, 118/400, 118/401, 118/407, 118/410, 118/411, 118/241, 118/243, 346/140.1, 346/141, 436/518, 436/180, 347/20, 347/21, 289/102, 289/102.2
• US Class Current: 400/124.28
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00369 in multiple or parallel arrangements
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00533 essentially rectangular
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00689 using computers
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00731 Saccharides
• CPC Class Codes: B01L 2200/022 Variable spacings
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 2400/025 tapping tip on substrate
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 3/0244 using pins
• CPC Class Codes: B01L 3/0251 Pin and ring type or pin in tube type dispenser
• CPC Class Codes: B41J 2/22 characterised by selective application of impact or pressure on a printing material or impression-transfer material
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 40/12 : Libraries containing saccharides or polysaccharides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

A pin-in-capillary apparatus for depositing droplets of fluid onto a surface is described. The apparatus has an off-axis channel for supplying fluid to the capillary. In the capillary the fluid wets the tip of the pin and is delivered to the surface when the pin is extended from the capillary. The capillaries and pins are flexible, allowing spatial reformatting for fluid delivery. Methods of delivering fluid to a surface are also described.

Claims

1. An apparatus for depositing a fluid from a fluid source onto a substrate, the apparatus comprising

an off-axis channel adapted to being fluidically coupled to said fluid source,

a flexible capillary in fluid communication with the off-axis channel, the capillary having a downstream capillary end adapted to receiving fluid from said fluid source,

a flexible pin slidably disposed within the capillary, the pin having a downstream tip disposed adjacent the downstream capillary end, the tip being adapted to being wetted by said fluid in the capillary, and

a pin driver operably coupled to the pin,

wherein the pin driver is operable to move the tip toward the substrate to deposit said fluid onto said substrate.

2. An apparatus according to claim 1 having a plurality of flexible pins and flexible capillaries, each flexible pin slidably disposed within a flexible capillary.

3. The apparatus of claim 2 wherein a plurality of flexible pins are coupled to a common pin driver, the pin driver operable to extend the flexible pins as a group.

4. The apparatus of claim 2 having a plurality of pin drivers, wherein each flexible pin is coupled to a separate pin driver, each pin driver operable to extend the flexible pin coupled to that pin driver independently of every other flexible pin.

5. The apparatus of claim 2 having a plurality of pin drivers, each pin driver operably coupled to a plurality of flexible pins, each pin driver capable of extending said plurality of flexible pins coupled to said driver as a group.

6. The apparatus of claim 2 wherein each flexible capillary has a uniform cross-sectional area along its length.

7. The apparatus of claim 2, further comprising a downstream capillary holder retaining the flexible capillaries at or adjacent to the downstream capillary ends in a defined downstream format.

8. The apparatus of claim 7, wherein the upstream capillary ends are retained by a manifold body retaining the upstream ends of the flexible capillaries in a defined upstream format.

9. The apparatus of claim 8 wherein the defined downstream format is different from the defined upstream format.

10. The apparatus of claim 8 wherein the defined downstream format has the flexible capillaries relatively closer together than in the defined upstream format.

11. The apparatus of claim 8 wherein the defined downstream format is scrambled with respect to the defined upstream format.

12. The apparatus of claim 1, wherein the flexible capillary has an upstream capillary end opposite the downstream capillary end, wherein the capillary defines a capillary lumen, and wherein the flexible pin runs from the tip through the capillary lumen to the pin driver.

13. The apparatus of claim 12 further comprising a flex region operably associated with the flexible pin.

14. The apparatus of claim 13 wherein the flex region is incorporated into the structure of the flexible pin.

15. The apparatus of claim 12 wherein the flexible pin has a flex region between the upstream capillary end and the pin driver.

16. The apparatus of claim 1 wherein the flexible capillary defines a capillary lumen and the capillary lumen may be supplied with fluid without removing the flexible pin from the flexible capillary.

17. The apparatus of claim 1, further comprising at least 12 apparatuses according to claim 1 held in a fixed arrangement with respect to each other.

18. The apparatus of claim 1, further comprising at least 96 apparatuses according to claim 1 held in a fixed arrangement with respect to each other.

19. The apparatus of claim 1, further comprising at least 384 apparatuses according to claim 1 held in a fixed arrangement with respect to each other.

20. The apparatus of claim 1, further comprising at least 1536 apparatuses according to claim 1 held in a fixed arrangement with respect to each other.