Device and method for determining the concentration of a substrate
First Claim
1. A biosensor for determining the concentration of a substrate in a sample solution, said biosensor comprising:
- an electrically insulating base plate;
an electrode system having a working electrode, a counter electrode and a third electrode to be utilized as an interfering substance detecting electrode, said working electrode, said counter electrode and said third electrode being formed on said base plate;
a reaction layer comprising an oxidoreductase and an electron mediator, said reaction layer being formed on said electrode system so as to cover said working electrode and said counter electrode, said reaction layer not covering said third electrode; and
a cover member forming a sample solution supply pathway to introduce a sample solution from a sample solution supply port into said reaction layer on said base plate, wherein said third electrode being located closer to said sample solution supply port than said reaction layer and said entire working electrode being located closer to said sample solution supply port than said counter electrode.
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Abstract
An apparatus for determining the concentration of a substrate in a sample solution using an electrode system comprising a working electrode, a counter electrode, and a reaction layer which contains at least an oxidoreductase and an electron mediator and is formed on the electrode system to electrochemically measure a reduced amount of the electron mediator resulting from enzyme reaction in the reaction layer, wherein a third electrode is formed as an interfering substance detecting electrode. A current flowing between the counter electrode and the third electrode is measured which is taken as a positive error. Subsequently, voltage application between the counter electrode and the third electrode is released and a voltage for oxidizing the reduced form electron mediator is applied between the working electrode and the counter electrode to measure a current flowing between the two electrodes.
180 Citations
5 Claims
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1. A biosensor for determining the concentration of a substrate in a sample solution, said biosensor comprising:
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an electrically insulating base plate;
an electrode system having a working electrode, a counter electrode and a third electrode to be utilized as an interfering substance detecting electrode, said working electrode, said counter electrode and said third electrode being formed on said base plate;
a reaction layer comprising an oxidoreductase and an electron mediator, said reaction layer being formed on said electrode system so as to cover said working electrode and said counter electrode, said reaction layer not covering said third electrode; and
a cover member forming a sample solution supply pathway to introduce a sample solution from a sample solution supply port into said reaction layer on said base plate, wherein said third electrode being located closer to said sample solution supply port than said reaction layer and said entire working electrode being located closer to said sample solution supply port than said counter electrode. - View Dependent Claims (2, 3, 4, 5)
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Specification