Systems and methods for cell subpopulation analysis
First Claim
1. A fluidic device for the analysis of cells, the device comprising:
- a dielectrophoretic field-flow fractionation separator configured to discriminate cells by balancing a dielectrophoretic force with a gravitational force to displace the cells to positions within a velocity profile in the separator; and
a multi-segment electrode array isolator coupled to the separator and configured to trap at least a portion of the cells emerging from the separator.
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Abstract
Systems, apparatuses and methods for cell isolation and analysis. Cells are introduced into a dielectrophoretic prefilter including one or more trapping electrodes configured to trap at least a portion of the cells with a dielectrophoretic force. The cells trapped from the prefilter are directed into a dielectrophoretic field-flow fractionation separator coupled to the prefilter. The cells are discriminated by balancing a dielectrophoretic force (and optionally a magnetophoretic force) with a gravitational force to displace the cells to positions within a velocity profile in the separator. At least a portion of the cells are trapped as a function of the cells'"'"' time of emergence from the separator with two or more spiral electrode segments coupled to the separator.
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Citations
32 Claims
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1. A fluidic device for the analysis of cells, the device comprising:
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a dielectrophoretic field-flow fractionation separator configured to discriminate cells by balancing a dielectrophoretic force with a gravitational force to displace the cells to positions within a velocity profile in the separator; and
a multi-segment electrode array isolator coupled to the separator and configured to trap at least a portion of the cells emerging from the separator. - View Dependent Claims (2, 3, 4, 5)
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6. A fluidic device for the analysis of cells, the device comprising:
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a dielectrophoretic prefilter comprising one or more trapping electrodes configured to trap at least a portion of the cells with a dielectrophoretic force;
a dielectrophoretic field-flow fractionation separator coupled to the prefilter and configured to discriminate cells by balancing a dielectrophoretic force with a gravitational force to displace the cells to positions within a velocity profile in the separator; and
two or more spiral electrode segments coupled to the separator and configured to trap at least a portion of the cells as a function of the cells'"'"' time of emergence from the separator. - View Dependent Claims (7, 8, 9, 10, 11)
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12. A fluidic device for the analysis of cells, the device comprising:
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a dielectrophoretic field-flow fractionation separator configured to discriminate cells by balancing a dielectrophoretic force with a gravitational force to displace the cells to positions within a velocity profile in the separator;
a multi-segment electrode array isolator coupled to the separator and configured to trap at least a portion of the cells emerging from the separator; and
a programmable fluidic processor coupled to the electrode array isolator. - View Dependent Claims (13, 14)
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15. A method for cell isolation and analysis, comprising:
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introducing cells into a dielectrophoretic field-flow fractionation separator;
discriminating the cells in the separator, the discriminating comprising balancing a dielectrophoretic force with a gravitational force to displace the cells to positions within a velocity profile in the separator; and
trapping at least a portion of the cells emerging from the separator with a multi-segment electrode array isolator coupled to the separator. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22)
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23. A method for cell isolation and analysis, comprising:
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introducing cells into a dielectrophoretic prefilter comprising one or more trapping electrodes configured to trap at least a portion of the cells with a dielectrophoretic force;
directing the cells trapped from the prefilter into a dielectrophoretic field-flow fractionation separator coupled to the prefilter;
discriminating the cells, the discriminating comprising balancing a dielectrophoretic force with a gravitational force to displace the cells to positions within a velocity profile in the separator; and
trapping at least a portion of the cells as a function of the cells'"'"' time of emergence from the separator with two or more spiral electrode segments coupled to the separator. - View Dependent Claims (24, 25, 26, 27, 28, 29)
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30. A method for cell isolation and analysis, comprising:
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introducing cells into a dielectrophoretic field-flow fractionation separator;
discriminating the cells in the separator, the discriminating comprising balancing a dielectrophoretic force with a gravitational force to displace the cells to positions within a velocity profile in the separator;
trapping at least a portion of the cells emerging from the separator with a multi-segment electrode array isolator coupled to the separator;
manipulating the cells with a programmable fluidic processor coupled to the electrode array isolator. - View Dependent Claims (31, 32)
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Specification