Methods of identifying and ADP-glucose receptor ligand, agonist or antagonist
First Claim
1. A method of identifying an ADP-glucose receptor agonist or antagonist, comprising:
- (a) contacting an ADP-glucose receptor polypeptide with at least one candidate compound in the presence of ADP-glucose under conditions wherein said receptor produces a G-protein coupled signal in response to ADP-glucose, wherein said ADP-glucose receptor polypeptide has the amino acid sequence designated SEQ ID NO;
2;
(b) determining the ability of said candidate compound to alter production of said G-protein coupled signal, wherein a candidate compound that alters production of said signal is identified as an ADP-glucose receptor agonist or antagonist.
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Abstract
The present invention provides an isolated nucleic acid molecule containing a nucleotide sequence which encodes an ADP-glucose receptor, and isolated polynucleotides therefrom. Also provided is an isolated ADP-glucose receptor polypeptide, an isolated immunogenic peptide therefrom, and antibodies specific therefor. The invention also provides a method of identifying an ADP-glucose receptor agonist or antagonist, by contacting an ADP-glucose receptor with one or more candidate compounds under conditions suitable for detection of a G-protein coupled signal in response to ADP-glucose, and identifying a candidate compound that alters production of the signal. Further provided is a method of identifying an ADP-glucose receptor ligand, by contacting an ADP-glucose receptor with one or more candidate compounds under conditions suitable for detecting selective binding of ADP-glucose to ADP-glucose receptor, and identifying a candidate compound that selectively binds the ADP-glucose receptor. Also provided are methods of diagnosing or determining susceptibility to ADP-glucose receptor associated conditions, by detecting in a sample from the individual expression of ADP glucose receptor nucleic acid molecules or polypeptides.
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Citations
20 Claims
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1. A method of identifying an ADP-glucose receptor agonist or antagonist, comprising:
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(a) contacting an ADP-glucose receptor polypeptide with at least one candidate compound in the presence of ADP-glucose under conditions wherein said receptor produces a G-protein coupled signal in response to ADP-glucose, wherein said ADP-glucose receptor polypeptide has the amino acid sequence designated SEQ ID NO;
2;
(b) determining the ability of said candidate compound to alter production of said G-protein coupled signal, wherein a candidate compound that alters production of said signal is identified as an ADP-glucose receptor agonist or antagonist. - View Dependent Claims (2, 3, 4, 5)
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6. A method of identifying an ADP-glucose receptor agonist, comprising:
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(a) contacting an ADP-glucose receptor polypeptide with at least one candidate compound under conditions that permit said receptor to produce a G-protein coupled signal in response to ADP-glucose, wherein said ADP-glucose receptor polypeptide has the amino acid sequence designated SEQ ID NO;
2; and
(b) determining the ability of said candidate compound to increase production of said G-protein coupled signal, wherein a candidate compound that increases production of said signal is thereby identified as an ADP-glucose receptor agonist. - View Dependent Claims (7, 8, 9, 10, 11)
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12. A method of identifying an ADP-glucose receptor ligand, comprising:
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(a) contacting an ADP-glucose receptor polypeptide with at least one candidate compound under conditions that permit said receptor to selectively bind ADP-glucose, wherein said ADP-glucose receptor polypeptide has the amino acid sequence designated SEQ ID NO;
2; and
(b) determining the ability of said candidate compound to bind said ADP glucose receptor, wherein a candidate compound that selectively binds said ADP-glucose receptor is thereby identified as an ADP-glucose receptor ligand. - View Dependent Claims (13, 14, 15, 16)
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17. A method of identifying an ADP-glucose receptor ligand, comprising:
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(a) contacting an ADP-glucose receptor polypeptide with at least one candidate compound in the presence of ADP-glucose under conditions that permit said receptor to selectively bind ADP-glucose, wherein said ADP-glucose receptor polypeptide has the amino acid sequence designated SEQ ID NO;
2; and
(b) determining the ability of said candidate compound to bind said ADP-glucose receptor, wherein a candidate compound that selectively binds said ADP-glucose receptor is thereby identified as an ADP-glucose receptor ligand. - View Dependent Claims (18, 19, 20)
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Specification