Method of preparing an oligonucleotide array
First Claim
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1. A method of making an oligonucleotide array comprising:
- a. providing an array of porous polymer gel pads on the surface of a solid support;
b. adding an oligonucleotide probe to each of said porous polymer gel pads;
c. freezing said array of porous polymer gel pads comprising said oligonucleotide probes to produce a frozen array said porous polymer gel pads being hydrated prior to freezing; and
d. drying said frozen array of porous polymer gel pads by sublimation for a time sufficient to increase the pore size of each of said porous polymer gel pads.
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Abstract
This invention relates to the improvement of arrays of porous polymer pads on solid supports used in biological assays. The invention involves freeze drying the porous polymer pads to increase pore size. The increased pore size results in an enhanced ability of the porous polymer pads to bind specific binding substances such as DNA, RNA and polypeptides.
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5 Claims
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1. A method of making an oligonucleotide array comprising:
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a. providing an array of porous polymer gel pads on the surface of a solid support;
b. adding an oligonucleotide probe to each of said porous polymer gel pads;
c. freezing said array of porous polymer gel pads comprising said oligonucleotide probes to produce a frozen array said porous polymer gel pads being hydrated prior to freezing; and
d. drying said frozen array of porous polymer gel pads by sublimation for a time sufficient to increase the pore size of each of said porous polymer gel pads. - View Dependent Claims (2, 3, 4, 5)
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Specification