Membrane receptor reagent and assay

• US 6,790,632 B2
• Filed: 05/23/2003
• Issued: 09/14/2004
• Est. Priority Date: 06/17/2002
• Status: Expired due to Fees

• Current Assignee: Stephen Eliot Zweig
• Original Assignee: Stephen Eliot Zweig
• Inventors: Zweig, Stephen Eliot
• Primary Examiner(s): Le, Long V.
• Assistant Examiner(s): YANG, NELSON C
• Application Number:
  US10/444,390
• Publication Number:
  US 20040033624A1
• Time in Patent Office: 480 Days
• Field of Search: 435/6-7, 436/165, 436/172, 436/518, 436/829, 436/164, 436/514, 436/523, 422/81-82, 204/403--40, 204/409-418, 205/775, 205/778, 250/364, 356/136, 356/128, 356/445, 356/519, 356/484, 356/301, 385/12, 385/37, 385/130
• US Class Current: 435/7.92
• CPC Class Codes: G01N 33/5432 Liposomes or microcapsules
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

A membrane receptor reagent and assay is disclosed in which liposomes are bound to an evanescent wave emitting surface. Membrane receptors on the liposome'"'"'s fluid lipid bilayer membrane are labeled with a fluorescent or luminescent moiety. These membrane receptors are free to diffuse randomly throughout the liposome surface, and thus tend to redistribute according to externally applied forces. The evanescent wave-emitting surface additionally contains reagents that reversibly bind to the membrane receptors, tending to bring them closer to region of high evanescent wave intensity. Test analytes that disrupt or promote the association between the membrane receptors and the surface reagents act to change the average distance between the membrane receptors and the evanescent wave emitting surface, resulting in a change in the fluorescent or luminescent signal. This reagent and assay system functions with physiologically important membrane receptors such as GPCR receptors, other 7-tm receptors, drug transport proteins, cytochrome P450 membrane proteins and other clinically important membrane components. The reagent and assay methods may be incorporated into microarrays, capillaries, flow cells and other devices, and used for drug discovery, ADMET, and other biomedically important assays.

Claims

1. A membrane-receptor reagent for measuring the interaction between test analytes and target membrane receptors comprising:

a support comprising an energy-emitting surface that emits energy that varies as a function of distance from said surface;

extended hydrophilic tethers attached to said energy-emitting surface of said support;

reagent ligands attached to the ends of said extended hydrophilic tethers;

a fluid lipid membrane in the form of a liposome, phospholipid vesicle, or lipid layers mounted on a second surface that projects away from said energy emitting surface, comprising target membrane receptors that specifically bind to target analytes and reversibly bind to said reagent ligands, wherein said target membrane receptors move fluidly within said fluid lipid membrane and further comprise integral or transmembrane membrane proteins labeled with a moiety that produces a detectable signal upon receiving excitation energy; and

anchoring means for irreversibly binding said fluid lipid membrane to said energy-emitting surface;

wherein binding between said target membrane receptors and target analyte either blocks or promotes binding between said reagent ligands and said target membrane receptors, causing a change in an average distance between said target membrane receptors and said energy-emitting surface, resulting in a change in said detectable signal.

2. The reagent of claim 1, in which said energy emitted by said surface is selected from the group consisting of evanescent waves, surface plasmon resonance, and electron transfer, and the detectable signal is selected from the group consisting of fluorescence, luminescence, electrochemiluminescence, electron transport, and surface plasmon resonance.

3. The reagent of claim 1, wherein said anchor means is selected from the group consisting of protein-ligand binding reagents, electrostatic interactions, hydrophobic interactions, polymeric tethers, and polymeric meshes.

4. The reagent of claim 1, wherein said target membrane receptors are membrane proteins selected from the group consisting of 7-transmembrane proteins, G-protein coupled receptors (GPCR), toll receptors, ion channels, ABC cassette pumps, hormone receptors, biological response modifier receptors, apoptosis receptors, angiogenesis receptors, neuroreceptors, histocompatibility antigens, coagulation factors, immune response antigens, and cytochrome P450 enzymes.

5. The reagent of claim 1, wherein said target membrane receptors and test analytes are selected from membrane receptors and analytes used in drug discovery, absorption distribution metabolism and excretion (ADME), toxicology, cellular proliferation, cellular regulation, and medical diagnostics assays.

6. The reagent of claim 1, further comprising a reference energy source, which generates a normalization signal representative of the number of said signal generating label moieties.

7. The reagent of claim 1, wherein said support is a microarray, capillary tube, fiber optic fiber, or flow cell device.

8. A membrane-receptor reagent for measuring the interaction between test analytes and target membrane receptors comprising:

a support comprising an energy-emitting surface that emits evanescent energy that varies as a function of distance from said surface;

extended hydrophilic tethers attached to said energy-emitting surface of said support;

reagent ligands attached to the ends of said extended hydrophilic tethers;

a fluid lipid membrane in the form of a liposome, phospholipid vesicle, or lipid layers mounted on a second surface that projects away from said energy emitting surface, comprising target membrane receptors that specifically bind to target analytes and reversibly bind to said reagent ligands, wherein said target membrane receptors move fluidly within said fluid lipid membrane and further comprise integral or transmembrane membrane proteins labeled with a moiety that produces a fluorescent or luminescent signal upon receiving evanescent energy; and

anchoring means for irreversibly binding said fluid lipid membrane to said energy-emitting surface;

wherein binding between said target membrane receptors and target analyte either blocks or promotes binding between said reagent ligands and said target membrane receptors, causing a change in an average distance between said target membrane receptors and said energy-emitting surface, resulting in a change in said fluorescent or luminescent signal.

9. The reagent of claim 8, wherein said target membrane receptors and test analytes are selected from membrane proteins and analytes used in drug discovery, absorption distribution metabolism and excretion (ADME), toxicology, cellular proliferation, cellular regulation, and medical diagnostic assays.

10. The reagent of claim 8, further comprising a reference energy source, which generates normalization signal representative of the number of said signal generating label moieties.

11. The reagent of claim 8, wherein said support is a microarray, capillary lube, fiber optic fiber, or flow cell device.

12. A method for measuring said interaction between test analytes and target membrane receptors, comprising the steps of:

a) providing a membrane-receptor reagent comprising;

a support comprising an energy-emitting surface that emits energy that varies as a function of distance from said surface;

extended hydrophilic tethers attached to said energy-emitting surface of said support;

reagent ligands attached to the ends of said extended hydrophilic tethers;

a fluid lipid membrane in the form of a liposome, phospholipid vesicle, or lipid layers mounted on a second surface that projects away from said energy emitting surface, comprising target membrane receptors that specifically bind to target analytes and reversible bind to said reagent ligands, wherein said target membrane receptors move fluidly within said fluid lipid membrane and further comprise integral or transmembrane membrane proteins labeled with a moiety that produces a detectable signal upon receiving, excitation energy; and

anchoring means for irreversibly binding said fluid lipid membrane to said energy-emitting surface;

b) measuring an initial detectable signal emitted from the signal generating label moieties in said reagent;

c) adding a sample comprising target analytes to said membrane-receptor reagent, wherein binding between said target membrane receptors and target analyte either blocks or promotes binding between said reagent ligands and target membrane, causing a change in the average distance between the target membrane receptors and the energy-emitting surface, resulting in a change in the detectable signal; and

d) measuring changes in said detectable signal after sample has been added, wherein said changes in said detectable signal indicates the interaction between said target analyte and said target membrane receptors.

13. The method of claim 12, wherein said energy-emitting surface emits evanescent waves, and said detectable signal is a fluorescent or luminescent signal.

14. The method of claim 12, in which the changes in the detectable signal are adjusted by a reference signal.

15. The method of claim 12, where said support is a microarray, capillary tube, fiber optic fiber, or flow cell device.

16. The method of claim 12, wherein said target membrane receptors and test analytes are selected from membrane receptors and analytes used in drug discovery, absorption distribution metabolism and excretion (ADME), toxicology, cellular proliferation, cellular regulation, and medical diagnostics assays.

17. The method of claim 12, wherein fluidic switch elements dynamically direct the passage of the test analytes to different assay devices.

18. A method for identifying a target drug molecule for a drug compound comprising the steps of:

a) providing a membrane-receptor reagent comprising;

a support comprising an energy-emitting surface that emits energy that varies as a function of distance from said surface;

extended hydrophilic tethers attached to said energy-emitting surface of said support;

reagent ligands attached to the ends of said extended hydrophilic tethers;

a fluid lipid membrane in the form of a liposome, phospholipid vesicle, or lipid layers mounted on a second surface that projects away from said energy emitting surface, comprising target membrane receptors that specifically bind to target drug molecules and reversibly bind to said reagent ligands, wherein said target membrane receptors move fluidly within said fluid lipid membrane and further comprise integral or transmembrane membrane proteins labeled with a moiety that produces a detectable signal upon receiving excitation energy, and

anchoring means for irreversibly binding said fluid lipid membrane to said energy-emitting surface,

b) measuring an initial detectable signal emitted from the signal generating label moieties in said reagent;

c) adding a sample comprising target drug molecules to said membrane-receptor reagent, wherein binding between said target membrane receptors and target analyte either blocks or promotes binding between said reagent ligands and target membrane, causing a change in the average distance between the target membrane receptors and the energy-emitting surface, resulting in a change in the detectable signal; and

d) measuring changes in said detectable signal after sample has been added, wherein said changes in said detectable signal indicates the amount of interaction between said target drug molecules and said target membrane receptors.