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Monitoring patient compliance and bioavailability of drugs by deproteinizing body fluids

  • US 6,790,668 B1
  • Filed: 09/07/2000
  • Issued: 09/14/2004
  • Est. Priority Date: 11/23/1998
  • Status: Expired due to Term
First Claim
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1. Method of monitoring patient compliance and bioavailability of drugs contained in a body fluid consisting essentially of the following steps:

  • (a) mixing and shaking mechanically the body fluid with a 0.1 M to 5.0 M aqueous zinc sulfate solution to precipitate proteins, and an appropriate solvent to extract the drug during deproteinizing, in a single step, and recovering at least 97% of the drug;

    (b) centrifuging the mixture of (a), to obtain the separation of phases;

    (c) recovering the supernatant of (b) and measuring the drug concentration in body fluid using a colorimetric assay or a High-Performance Liquid Chromatography method, and (d) obtaining drug levels down to at least 0.3 μ

    g/ml.

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