LKB1 gene knockout mice

• US 6,791,006 B2
• Filed: 11/30/2001
• Issued: 09/14/2004
• Est. Priority Date: 05/31/1999
• Status: Expired due to Fees

• Current Assignee: Chugai Seiyaku Kabushiki Kaisha (Roche Holding AG)
• Original Assignee: Chugai Seiyaku Kabushiki Kaisha (Roche Holding AG)
• Inventors: Nezu, Jun-Ichi, Ose, Asuka, Jishage, Kou-Ichi, Jenne, Dieter E.
• Primary Examiner(s): Crouch, Deborah
• Assistant Examiner(s): BERTOGLIO, VALARIE E
• Application Number:
  US10/006,611
• Publication Number:
  US 20020166137A1
• Time in Patent Office: 1,019 Days
• Field of Search: 800/14, 800/18, 800/21, 800/22, 800/25
• US Class Current: 800/18
• CPC Class Codes: A01K 2217/072 maintaining or altering function, i.e. knock in
• CPC Class Codes: A01K 2217/075 inducing loss of function, i.e. knock out
• CPC Class Codes: A01K 2227/10 Mammal
• CPC Class Codes: A01K 2227/105 Murine
• CPC Class Codes: A01K 2267/0306 Animal model for genetic diseases
• CPC Class Codes: A01K 2267/0331 Animal model for proliferative diseases
• CPC Class Codes: A01K 2267/0393 Animal model comprising a reporter system for screening tests
• CPC Class Codes: A01K 67/0276 Knock-out vertebrates
• CPC Class Codes: C12N 15/8509 for producing genetically modified animals, e.g. transgenic
• CPC Class Codes: C12N 15/902 using homologous recombination
• CPC Class Codes: C12N 15/907 in mammalian cells
• CPC Class Codes: C12N 2800/30 Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
• CPC Class Codes: C12N 2830/00 Vector systems having a special element relevant for transcription
• CPC Class Codes: C12N 9/1205 Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases

Abstract

A mammal is provided, in which the LKB1 gene can be deleted phase-specifically and tissue-specifically. These mammals are highly useful as tools to reveal the onset mechanism for diseases caused by LKB1 gene deficiency, such as Peutz-Jeghers syndrome and cancers, as well as to develop therapeutic agents, methods, and so on for the diseases.

Claims

1. A transgenic postnatal mouse whose genome comprises a heterozygous disruption in the LKB1 gene as a result of recombinase mediated gene disruption prior to embryogenesis, wherein the mouse exhibits digestive tract polyposis.

2. The transgenic postnatal mouse of claim 1, wherein the recombinase is Cre.

3. The transgenic postnatal mouse of claim 1, wherein the recombinase is Flp.