Flow cytometer

• US 6,793,642 B2
• Filed: 07/30/2001
• Issued: 09/21/2004
• Est. Priority Date: 05/07/2001
• Status: Expired due to Fees

• Current Assignee: Biomed Solutions, LLC
• Original Assignee: Biomed Solutions, LLC
• Inventors: Gray, Robert W., MacDonald, Stuart G., Connelly, Patrick R., Gebert, Carol
• Primary Examiner(s): Sykes, Angela D.
• Assistant Examiner(s): Deak, Leslie R.
• Application Number:
  US09/918,076
• Publication Number:
  US 20030036718A1
• Time in Patent Office: 1,149 Days
• Field of Search: 604/4.01, 604/5.01, 604/5.02, 604/5.04, 604/6.08, 604/6.09, 604/6.11, 604/265, 210/668, 210/669, 422/82.09, 600/309, 600/310, 600/342, 600/407, 600/473, 600/476, 356/335-338, 435/4, 435/238.1, 424/489, 436/518
• US Class Current: 604/5.01
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence
• CPC Class Codes: A61B 5/076 Permanent implantations telemetry using implanted circuitry A61B5/0031; implanted stimulators for therapy A61N1/3605, A61N1/362, A61N1/372
• CPC Class Codes: A61B 5/145 Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissuemeasuring of blood pressure or blood flow A61B5/02; non-radiation detecting or locating of foreign bodies in blood A61B5/06
• CPC Class Codes: A61B 5/150015 Source of blood
• CPC Class Codes: A61B 5/150221 Valves
• CPC Class Codes: A61B 5/150229 Pumps for assisting the blood sampling
• CPC Class Codes: A61B 5/417 the bone marrow
• CPC Class Codes: A61F 2/82 Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents stent-grafts for tubular structures of the body other than blood vessels A61F2/04; stent-grafts for blood vessels A61F2/07; instruments specially adapted for placement or removal of stents or stent-grafts A61F2/95; for closing wounds, or holding wounds closed A61B17/04 - A61B17/115; dilators A61M29/00
• CPC Class Codes: A61M 1/3621 Extra-corporeal blood circuits single-needle circuits A61M1/30
• CPC Class Codes: A61M 1/3679 by absorption A61M1/3675 takes precedence
• CPC Class Codes: A61M 1/3687 Chemical treatment A61M1/3675 takes precedence
• CPC Class Codes: A61M 1/38 Removing constituents from donor blood and storing or returning remainder to body , e.g. for transfusion
• CPC Class Codes: G01N 15/10 Investigating individual particles
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2015/1006 for cytology

Abstract

A flow cytometer containg a device for sampling cellular material within a body, comprising. The flow cytometer is adapted to mark cells within bodily fluid with a marker to produce marked cells, to analyze the marked cells, to sort the cells within the bodily fluid to produce sorted cells, and to maintain the sorted cells cells in a viable state.

Claims

1. A flow cytometer comprising means for sampling cellular material within a body comprised of blood, means for marking cells within said blood with a dye to produce marked cells, an analyzer for analyzing said marked cells, a dye stripper for removing said dye from said marked cells, a cell sorter for sorting said cells within said blood to produce sorted cells, and a reservoir comprising a permeable membrane for maintaining a first fraction of said sorted cells in a viable state.

2. The flow cytometer as recited in claim 1, further comprising means for removing said first fraction from said reservoir.

3. The flow cytometer as recited in claim 1, comprising means for returning said first fraction to said body.