Microfluidic method employing delivery of plural different fluids to same lumen

• US 6,797,056 B2
• Filed: 01/29/2002
• Issued: 09/28/2004
• Est. Priority Date: 06/08/2001
• Status: Expired due to Fees

• Current Assignee: Takeda San Diego, Inc. (Takeda Pharmaceutical Company Limited)
• Original Assignee: Syrrx Inc. (Takeda Pharmaceutical Company Limited)
• Inventors: David, Peter R.
• Primary Examiner(s): KUNEMUND, ROBERT M
• Application Number:
  US10/060,955
• Publication Number:
  US 20020195050A1
• Time in Patent Office: 973 Days
• Field of Search: 117/68, 117/69, 117/70, 117/201, 117/202, 117/206, 117/927, 422/245.1, 232/95.R
• US Class Current: 117/68
• CPC Class Codes: B01J 2219/00274 Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
• CPC Class Codes: B33Y 80/00 Products made by additive manufacturing
• CPC Class Codes: C30B 29/58 Macromolecular compounds
• CPC Class Codes: C30B 7/00 Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions from molten solvents C30B9/00; by normal or gradient freezing C30B11/00; under a protective fluid C30B27/00
• CPC Class Codes: G01N 25/147 by cristallisation
• CPC Class Codes: Y10T 117/1004 with means for measuring, testing, or sensing
• CPC Class Codes: Y10T 117/1008 with responsive control means
• CPC Class Codes: Y10T 117/1024 for crystallization from liquid or supercritical state

Abstract

A microfluidic method is provided that comprises: delivering first and second fluids to a lumen of a microfluidic device such that the first and second fluids flow adjacent to each other within the lumen without mixing except for diffusion at an interface between the first and second fluids, wherein the first fluid is different than the second fluid.

Claims

1. A microfluidic method comprising:

delivering first and second fluids to a lumen of a microfluidic device such that the first and second fluids flow adjacent to each other within the lumen without mixing except for diffusion at an interface between the first and second fluids, wherein the first fluid is different than the second fluid and the composition of at least one of the first and second fluids varies over time as it is delivered to the lumen so that the fluid forms a gradient with regard to a concentration of at least one component of the fluid that changes along a length of the lumen.

2. A microfluidic method according to claim 1 wherein the microfluidic device comprises a plurality of lumens, the method comprising delivering first and second fluids to each of the plurality of lumens.

3. A microfluidic method according to claim 1 wherein the same first and second fluids are delivered to each of the plurality of lumens.

4. A microfluidic method according to claim 1 wherein different first and second fluids are delivered to the different lumens of the plurality of lumens.

5. A microfluidic method according to claim 1 wherein the lumen has a cross sectional diameter of less than 2.5 mm.

6. A microfluidic method according to claim 1 wherein the lumen has a cross sectional diameter of less than 1 mm.

7. A microfluidic method according to claim 1 wherein the lumen has a cross sectional diameter of less than 500 microns.

8. A microfluidic method according to claim 1 wherein the first and second fluids combine to form different crystallization conditions for crystallizing a molecule.

9. A microfluidic method according to claim 1 wherein the first and second fluids combine to form different crystallization conditions for crystallizing a protein.

10. A microfluidic method according to claim 1 wherein the first and second fluids combine to form different crystallization conditions for crystallizing a macromolecule with a molecular weight of at least 500 Daltons.

11. A microfluidic method according to claim 1 wherein the first and second fluids combine to form different crystallization conditions for crystallizing a member selected from the group consisting of viruses, proteins, peptides, nucleosides, nucleotides, ribonucleic acids, deoxyribonucleic acids.

12. The method according to claim 1 wherein the material to be crystallized contains at least two or more materials selected from the group consisting of viruses, proteins, peptides, nucleosides, nucleotides, ribonucleic acids, deoxyribonucleic acids, small molecules, drugs, putative drugs, inorganic compounds, metal salts, organometallic compounds and elements.

13. A microfluidic method according to claim 1 wherein the first and second fluids have a same flow rate within the lumen.

14. A microfluidic method according to claim 1 wherein the first and second fluids have a different flow rate within the lumen.

15. A microfluidic method comprising:

delivering first and second fluids to a lumen of a microfluidic device such that the first and second fluids flow adjacent to each other within the lumen without mixing except for diffusion at an interface between the first and second fluids, wherein the first fluid is different than the second fluid and a composition of at least one of the first and second fluids delivered to the lumen is varied so that the composition of at least one of the first and second fluids within the lumen varies along a length of the lumen.

16. A microfluidic method comprising:

delivering first, second and third fluids to a lumen of a microfluidic device such that the first, second and third fluids flow adjacent to each other within the lumen without mixing except for diffusion at an interface between the first, second and third fluids, wherein the first, second and third fluids are different than each other and a composition of at least one of the first, second and third fluids delivered to the lumen is varied so that the composition of at least one of the first, second, and third fluids within the lumen varies along a length of the lumen.

17. A microfluidic method according to claim 16 wherein the composition of at least one of the first, second and third fluids varies over time as it is delivered to the lumen so that the fluid forms a gradient with regard to a concentration of at least one component of the fluid that changes along a length of the lumen.

18. A microfluidic method according to claim 16 wherein the microfluidic device comprises a plurality of lumens, the method comprising delivering first, second and third fluids to each of the plurality of lumens.

19. A microfluidic method according to claim 16 wherein the same first, second and third fluids are delivered to each of the plurality of lumens.

20. A microfluidic method according to claim 16 wherein different first, second, and third fluids are delivered to the different lumens of the plurality of lumens.

21. A microfluidic method according to claim 16 wherein the lumen has a cross sectional diameter of less than 2.5 mm.

22. A microfluidic method according to claim 16 wherein the lumen has a cross sectional diameter of less than 1 mm.

23. A microfluidic method according to claim 16 wherein the lumen has a cross sectional diameter of less than 500 microns.

24. A microfluidic method according to claim 16 wherein at least one of the first, second and third fluids have a different flow rate than another of the fluids within the lumen.

25. A microfluidic method according to claim 16 wherein at least one of the first, second and third fluids have a same flow rate than another of the fluids within the lumen.

26. A microfluidic method according to claim 16 wherein the first, second and third fluids combine to form different crystallization conditions.

27. A microfluidic method according to claim 16 wherein the first, second and third fluids combine to form different crystallization conditions, the second fluid comprising the material to be crystallized and being positioned between the first and third fluids.

28. A microfluidic method according to claim 16 wherein the first, second and third fluids combine to form different crystallization conditions for crystallizing a molecule.

29. A microfluidic method according to claim 16 wherein the first, second and third fluids combine to form different crystallization conditions for crystallizing a protein.

30. A microfluidic method according to claim 16 wherein the first, second and third fluids combine to form different crystallization conditions for crystallizing a macromolecule with a molecular weight of at least 500 Daltons.

31. A microfluidic method according to claim 16 wherein the first, second and third fluids combine to form different crystallization conditions for crystallizing a member selected from the group consisting of viruses, proteins, peptides, nucleosides, nucleotides, ribonucleic acids, deoxyribonucleic acids.

32. The method according to claim 16 wherein the material to be crystallized contains at least two or more materials selected from the group consisting of viruses, proteins, peptides, nucleosides, nucleotides, ribonucleic acids, deoxyribonucleic acids, small molecules, drugs, putative drugs, inorganic compounds, metal salts, organometallic compounds and elements.