Apparatus and process for the lateral deflection and separation of flowing particles by a static array of optical tweezers

• US 6,797,942 B2
• Filed: 09/13/2001
• Issued: 09/28/2004
• Est. Priority Date: 09/13/2001
• Status: Expired due to Fees

• Current Assignee: University of Chicago The (University of Chicago)
• Original Assignee: University of Chicago
• Inventors: Korda, Pamela T., Grier, David G.
• Primary Examiner(s): Lee, John R.
• Assistant Examiner(s): Hashmi, Zia R.
• Application Number:
  US09/951,117
• Publication Number:
  US 20030047676A1
• Time in Patent Office: 1,111 Days
• Field of Search: 250/251, 359/566, 359/15, 359/434, 359/9, 359/16, 436/518, 436/534, 436/524
• US Class Current: 250/251
• CPC Class Codes: B01D 2015/3895 using light
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 30/00 Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation G01N3/00, G01N5/00, G01N7/00, G01N9/00, G01N11/00, G01N13/00, G01N15/00, G01N17/00, G01N19/00, G01N21/00, G01N22/00, G01N23/00, G01N24/00, G01N25/00, G01N27/00, G01N29/00 take precedence
• CPC Class Codes: G01N 30/02 Column chromatography
• CPC Class Codes: G03H 2001/0077 for optical manipulation, e.g. holographic optical tweezers [HOT]

Abstract

A method and apparatus for laterally deflecting and/or separating a flow of particles using a static array of optical tweezers. In an array of optical tweezers with a lattice constant larger than the size of a particle of interest, particles driven past the array by an external force experience an additional interaction with the array of traps. By altering the angle of the array of traps relative to the external force, the particles'"'"' movement from trap to trap inside the array can be biased away from the direction of the external force, thereby enabling selective deflection and/or separation of particles.

Claims

1. An apparatus for controlled deflection of particles, comprising:

a source for a plurality of particles

an external bias force from a bias force source applied to the plurality of particles; and

an optical tweezer array including a plurality of optical tweezers having a trapping force associated with each of the tweezers, the optical tweezer array oriented at a tilt angle relative to a direction of the bias force generated by the bias force source.

2. The apparatus as defined in claim 1 wherein the tilt angle is selected to optimize flow velocity of the particles.

3. The apparatus as defined in claim 1 wherein the optical tweezer array comprises a plurality of optical potential wells, each having a specified optical intensity for establishing a desired potential well depth and arranged in a pattern for establishing a selected alignment relative to the external bias force.

4. The apparatus as defined in claim 3 wherein the optical tweezer array comprises at least two different potential well depths for manipulating different size particles using the plurality of optical tweezers.

5. The apparatus as defined in claim 1 wherein the tilt angle is adjustable to select direction of deflection of the particles.

6. The apparatus as defined in claim 5 wherein the angle of deflection varies with at least one of size of the particles, index of refraction of the particles relative to that of a medium in which the particles are disposed, shape of the particles, density of the particles, dielectric constant, magnetic susceptibility, and surface chemistry of the particles.

7. The apparatus as defined in claim 1 wherein the external bias force is adjusted to modify a deflection angle of the particles from the direction of the external force.

8. The apparatus as defined in claim 1 wherein control of particle motion is obtained by a laser beam source having varying laser light intensity for the formation of the optical tweezers.

9. The apparatus as defined in claim 8 wherein larger ones of the particles can be preferentially separated from smaller ones of the particles by selecting an appropriate laser light intensity.

10. The apparatus as defined in claim 1 further including additional stages of the optical tweezer array to further deflect the particles.

11. The apparatus as defined in claim 1 wherein the particles are selected from the group consisting of colloidal particles, macromolecules, biological cells, biological organelles, chromosomes, and mixtures thereof.

12. A method of controlling particle motion, comprising the steps of:

providing a flow of particles;

applying an external bias force to the particles; and

creating an optical tweezer array by forming an array of optical gradient fields with the optical tweezer array tilted at an angle relative to a direction of the external bias force.

13. The method as defined in claim 12 further including the steps of changing laser beam intensity used to create the optical tweezer array, thereby controlling flow of the particles.

14. The method as defined in claim 12 further including the steps of changing strength of the external bias force thereby controlling flow of the particles.

15. The method as defined in claim 12 wherein the particles have a range of at least one of size, shape, density, electronic charge, magnetic susceptibility, magnetic moment, dielectric constant, and linear and nonlinear optical properties which have variable response to at least one of the external bias force, laser beam intensity and optical tweezer array, thereby creating a range of deflection angles for the particles.

16. The method as defined in claim 12 wherein the angle is adjusted to change flow velocity of the particles.

17. The method as defined in claim 12 wherein deflection occurs continuously.

18. The method as defined in claim 12 wherein laser light is provided to form the optical tweezer array and wavelength of the laser light is adjusted to control particle motion.

19. The method as defined in claim 12 wherein at least one of the optical tweezer array, the external bias force and angle of tilt are changed dynamically as the particles flow.

20. The method as defined in claim 12 wherein the particles are selected from the group consisting of colloidal particles, macromolecules, biological cells, biological organelles, chromosomes, and mixtures thereof.