Apparatus and method for inactivating viral contaminants in body fluids
First Claim
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1. A method for inactivating viruses in a blood or blood component, the method comprising the steps of:
- initially storing an amount of methylene blue and an amount of the blood or blood component in separate sterile containers, each container having an inner surface made of a non-polyvinyl chloride, plastic material and the container in which the blood or blood component is stored includes portions that are made of a polyvinyl chloride material;
forming a mixture by adding at least a portion of the amount of the methylene blue to the amount of the blood or blood component wherein the portion of methylene blue is a virucidally effective amount of the methylene blue and further wherein the mixture formed is within one of the containers in which the methylene blue or blood or blood component is initially stored under sterile conditions; and
irradiating the mixture with a light field of a suitable intensity and wavelength for activating the methylene blue for a time sufficient to inactivate viruses in the mixture, while maintaining the mixture under a substantially no flow condition within the container in which the mixture is formed.
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Abstract
A method and apparatus for inactivating viruses in a body fluid. A mixture including a therapeutically effective amount of methylene blue and an amount of a body fluid is formed in a container under sterile conditions and then the container is irradiated with a light field of a suitable intensity and wavelength for activating the methylene blue for a time sufficient to inactivate viruses in the mixture. The mixture is maintained in a substantially static state within the container.
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Citations
18 Claims
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1. A method for inactivating viruses in a blood or blood component, the method comprising the steps of:
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initially storing an amount of methylene blue and an amount of the blood or blood component in separate sterile containers, each container having an inner surface made of a non-polyvinyl chloride, plastic material and the container in which the blood or blood component is stored includes portions that are made of a polyvinyl chloride material;
forming a mixture by adding at least a portion of the amount of the methylene blue to the amount of the blood or blood component wherein the portion of methylene blue is a virucidally effective amount of the methylene blue and further wherein the mixture formed is within one of the containers in which the methylene blue or blood or blood component is initially stored under sterile conditions; and
irradiating the mixture with a light field of a suitable intensity and wavelength for activating the methylene blue for a time sufficient to inactivate viruses in the mixture, while maintaining the mixture under a substantially no flow condition within the container in which the mixture is formed. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method for inactivating viruses in a blood or blood component, the method comprising the steps of:
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initially storing an amount of methylene blue having a pH of less than 7.0 in a container that includes an interior and at least one port extending from the container that provides controlled access to the interior, the container is constructed of a plastic material and includes an inner surface made of a non-polyvinyl chloride, plastic material;
forming a mixture by adding at least a portion of the amount of the methylene blue to an amount of the blood or blood component, by use of the port, wherein the portion of methylene blue is a virucidally effective amount of the methylene blue and further wherein the mixture formed is within either the container or in a second container that initially housed the blood or blood components; and
irradiating the mixture with a light field of a suitable intensity and wavelength for activating the methylene blue for a time sufficient to inactivate viruses in the mixture, while maintaining the mixture under a substantially no flow condition within the container in which the mixture is formed. - View Dependent Claims (9, 10, 11, 12, 13)
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14. A method for inactivating viruses in a blood or blood component, the method comprising the steps of:
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initially storing an amount of methylene blue in a container that includes an interior and at least one port extending from the container that provides controlled access to the interior, the container is constructed of a plastic material and includes an inner surface made of a non-polyvinyl chloride, plastic material;
forming a mixture bv adding at least a portion of the amount of the methylene blue to an amount of the blood or blood component, by use of the port, wherein the portion of methylene blue is a virucidally effective amount of the methylene blue and further wherein the mixture formed is within either the container or in a second container that initially housed the blood or blood component;
irradiating the mixture with a light field of a suitable intensity and wavelength for activating the methylene blue for a time sufficient to inactivate viruses in the mixture, while maintaining the mixture under a substantially no flow condition within the container in which the mixture is formed;
wherein the container in which the mixture is formed does not include any polyvinyl chloride plastic.
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15. A method for inactivating viruses in a blood or blood component, the method comprising the steps of:
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initially storing an amount of methylene blue in a container that includes an interior and at least one port extending from the container that provides controlled access to the interior, the container is constructed of a plastic material and includes an inner surface made of a non-polyvinyl chloride, plastic material;
forming a mixture by adding the blood or blood component to the container that contains methylene blue, by use of the port, wherein the portion of methylene blue is a virucidally effective amount of the methylene blue; and
irradiating the mixture with a light field of a suitable intensity and wavelength for activating the methylene blue for a time sufficient to inactivate viruses in the mixture, while maintaining the mixture under a substantially no flow condition within the container in which the mixture is formed.
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16. A method for inactivating viruses in a blood or blood component, the method comprising the steps of:
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initially storing an amount of methylene blue in a container that includes an interior and at least one port extending from the container that provides controlled access to the interior, the container is constructed of a plastic material and includes an inner surface made of a non-polyvinyl chloride, plastic material;
forming a mixture by adding at least a portion of the amount of the methylene blue to an amount of the blood or blood component, by use of the port, wherein the portion of methylene blue is a virucidally effective amount of the methylene blue and further wherein the mixture formed is within either the container or in a second container that initially housed the blood or blood component; and
irradiating the mixture with a light field of a suitable intensity and wavelength for activating the methylene blue for a time sufficient to inactivate viruses in the mixture, while maintaining the mixture under a substantially no flow condition within the container in which the mixture is formed;
wherein the container in which the mixture is formed comprises at least an inner surface made of a non-polyvinyl chloride material.
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17. A method for inactivating viruses in a blood or blood component the method comprising the steps of:
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initially storing an amount of methylene blue in a container that includes an interior and at least one port extending from the container that provides controlled access to the interior, the container is constructed of a plastic material and includes an inner surface made of a non-polyvinyl chloride, plastic material;
forming a mixture by adding at least a portion of the amount of the methylene blue to an amount of the blood or blood component, by use of the port, wherein the portion of methylene blue is a virucidally effective amount of the methylene blue and further wherein the mixture formed is within either the container or in a second container that initially housed the blood or blood component;
transporting the mixture in the container in which the mixture is formed on a conveyor past at least two light sources; and
irradiating the mixture with a light field of a suitable intensity and wavelength for activating the methylene blue for a time sufficient to inactivate viruses in the mixture, while maintaining the mixture under a substantially no flow condition within the container in which the mixture is formed.
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18. A method for inactivating viruses in a blood or blood component, the method comprising the steps of:
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initially storing an amount of methylene blue in a container that includes an interior and at least one port extending from the container that provides controlled access to the interior, the container is constructed of a plastic material and includes an inner surface made of a non-polyvinyl chloride, plastic material;
forming a mixture by adding at least a portion of the amount of the methylene blue to an amount of the blood or blood component, by use of the port, wherein the portion of methylene blue is a virucidally effective amount of the methylene blue and further wherein the mixture formed is within either the container or in a second container that initially housed the blood or blood component;
allowing excess methylene blue to leach into the container in which the blood or blood component is stored after mixture with the methylene blue; and
irradiating the mixture with a light field of a suitable intensity and wavelength for activating the methylene blue for a time sufficient to inactivate viruses in the mixture, while maintaining the mixture under a substantially no flow condition within the container in which the mixture is formed.
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Specification