Mammalian cytokine complexes

• US 6,800,460 B1
• Filed: 03/09/2000
• Issued: 10/05/2004
• Est. Priority Date: 03/11/1999
• Status: Expired due to Fees

• Current Assignee: Schering Corporation (Merck & Co., Inc.)
• Original Assignee: Schering Corporation (Merck & Co., Inc.)
• Inventors: Bazan, J. Fernando, Kastelein, Robert A., Oppmann, Birgit, Timans, Jacqueline C.
• Primary Examiner(s): Kunz, Gary
• Assistant Examiner(s): HAYES, ROBERT CLINTON
• Application Number:
  US09/521,335
• Time in Patent Office: 1,671 Days
• Field of Search: 536/23.5, 435/320.1, 435/325, 435/252.3, 435/69.1, 435/69.5, 435/69.52
• US Class Current: 435/69.5
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/54 Interleukins [IL]

Abstract

Purified genes encoding cytokine from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this molecule are provided. Methods of using said reagents and diagnostic kits are also provided.

Claims

1. An isolated or non-human host cell transfected with a first expression vector comprising a first nucleic acid encoding a first polypeptide of SEQ ID NO:

2 and a second expression vector comprising a second nucleic add encoding a second polypeptide of SEQ ID NO;

12.

2. The host cell of claim 1, wherein the host cell is:

a) a prokaryotic cell;

b) a mammalian cell;

c) an Insect cell;

or

d) a yeast cell.

3. A method of producing a soluble polypeptide complex of SEQ ID NO:

2 and SEQ ID NO;

12 comprising;

a) culturing the host cell of claim 1 under conditions suitable for expression of the first and second polypeptide;

b) allowing the polypeptide complex to form; and

c) isolating or purifying the soluble polypeptide complex.

4. An expression vector comprising:

a) a first nucleic acid encoding a first polypeptide of SEQ ID NO;

2 operably linked to a first promoter; and

b) a second nucleic acid encoding a second polypeptide of SEQ ID NO;

12 operably linked to a second promoter.

5. An isolated or non-human host cell comprising the expression vector of claim 4.

6. The host cell of claim 5, wherein the host cell is:

a) a prokaryotic cell;

b) a mammalian cell;

c) an insect cell;

or

d) a yeast cell.

7. A method of producing a soluble polypeptide complex of SEQ ID NO:

2 and SEQ ID NO;

12 comprising;

a) culturing the host cell of claim 5 under conditions suitable for expression of the first polypeptide and the second polypeptide;

b) allowing the polypeptide complex to form; and

c) isolating or purifying the soluble polypeptide complex.