Measurement of an analyte concentration in a scattering medium

• US 6,801,316 B2
• Filed: 07/19/2002
• Issued: 10/05/2004
• Est. Priority Date: 07/19/2002
• Status: Expired due to Fees

• Current Assignee: Optix LP
• Original Assignee: Optix LP
• Inventors: Guthermann, Howard E.
• Primary Examiner(s): Rosenberger, Richard A.
• Application Number:
  US10/198,889
• Publication Number:
  US 20040012789A1
• Time in Patent Office: 809 Days
• Field of Search: 356/39, 356/405, 356/437, 250/343, 600/310, 600/316, 600/322
• US Class Current: 356/437
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: G01N 21/359 using near infrared light
• CPC Class Codes: G01N 21/49 within a body or fluid

Abstract

A non-invasive detection method for glucose and other constituents of interest in a sample is described. The apparatus and methods of the invention provide an analog of color perception of human vision, preferably in the near infrared region, replacing the spectrophotometers used in other non-invasive near infrared detection methods. A plurality of detection units are used, each covering a broad region of the detected spectrum, paralleling color perception and colorimetry. In some circumstances, a neural net is used for analysis, allowing the system to learn. Analyte concentrations in scattering mediums can be obtained by calibrating the results with in vitro measurements.

Claims

1. A method for measuring the concentration of an analyte in a scattering medium, the method comprising the steps of:

illuminating a sample containing said analyte with broad spectrum radiation;

detecting an amount of transmitted, transflected, or reflected radiation from the sample with a detector;

associating a vector in a vector space with the amount of transmitted, transflected, or reflected radiation;

determining a correction vector from a measurement of a standard in a non-scattering medium as compared with the measurement of the same standard in the scattering medium; and

utilizing said correction vector and said vector associated with the amount of transmitted, transflected, or reflected radiation to identify and to obtain the concentration of the analyte in the scattering medium.

2. The method of claim 1, wherein said detecting step includes impinging the radiation transmitted, transflected, or reflected from the sample onto the detector that includes a plurality of detection units each responsive to a specific region of a spectrum associated with the transmitted, transflected, or reflected radiation, the spectral response of each of said detection units having at least partial overlap with the response of at least one other of said detection units.

3. The method of claim 2, wherein at least three detection units are used in said detecting step, each detection unit having a peak spectral response in a separate portion of said spectrum.

5. The method of claim 1, wherein said detector comprises broad bandpass filters.

6. The method of claim 1, wherein said detecting step further comprises the use of a black/white detection unit which is responsive to the entire spectrum for which said detector has a spectral response.

7. The method of claim 1, wherein the step of utilizing includes utilizing an artificial analog of a neural network.

8. The method of claim 7, wherein said artificial neural network is calibrated and trained to process said signals to achieve an analog of color constancy in vision.

9. The method of claim 1, and wherein said method is used to non-invasively determine the concentration of the analyte in a human body.

10. The method of claim 1, wherein said analyte is selected from the group consisting of glucose, glucose indicating constituents, hemoglobin, and hemoglobin indicating constituents.

11. The method of claim 1, wherein said analyte is selected from the group consisting of drugs of abuse and drugs of abuse indicating constituents.

12. The method of claim 1, wherein the sample includes a portion of a finger.

13. The method of claim 1, wherein the correction vector is obtained by obtaining a first vector associated with a first spectral property of the standard in the non-scattering medium;

and

obtaining a second vector associated with a second spectral property of the standard in a second sample containing the scattering medium.

14. The method of claim 13, wherein the first vector and the second vector both belong to a vector space whose dimension is equal to the number of detection units in the detection array.

15. The method of claim 14, wherein the step of utilizing includes calculating a rotation that transforms the second vector into the first vector, said rotation specifying the correction vector.

16. The method of claim 13, wherein the second sample is in vivo.

17. The method of claim 1, wherein the standard in the non-scattering medium is in vitro.

18. The method of claim 1, wherein the sample is in vivo.

4. The method of claim I wherein said detecting step includes impinging the radiation transmitted, transflected, or reflected from the sample onto the detector that includes a plurality of filters, each of said filters allowing transmission of a specific region of a spectrum associated with the transmitted, transflected, or reflected radiation, the spectral response of each of said filters having at least partial overlap with the response of at least one other of said filters, wherein each of said filters is impinged on the radiation transmitted, transflected, or reflected from said sample or standard sequentially and the output from said detector is used to measure the concentration.

19. The method of claim I, wherein the vector space has a dimension that is equal to the number of detection units.

20. A system for measuring the concentration of an analyte in a scattering medium, the system comprising:

a light source for illuminating a sample containing said analyte with broad spectrum radiation;

a detector for detecting an amount of transmitted, transflected, or reflected radiation from the sample;

a generator for generating a vector in a vector space that is associated with the amount of transmitted, transflected, or reflected radiation; and

an analyzer for determining a correction vector from a measurement of a standard in a non-scattering medium as compared with the measurement of the same standard in the scattering medium, and for utilizing said correction vector and said vector associated with the amount of transmitted, transflected, or reflected radiation to identify and to obtain the concentration of the analyte in the scattering medium.

21. The system of claim 20, wherein the detector includes a plurality of detection units each responsive to a specific region of a spectrum associated with the transmitted, transflected, or reflected radiation, the spectral response of each of said detection units having at least partial overlap with the response of at least one other of said detection units.

22. The system of claim 21, wherein the detector includes at least three detection units, each detection unit having a peak spectral response in a separate portion of said spectrum.

23. The system of claim 20, wherein the detector includes a plurality of filters, each of said filters allowing transmission of a specific region of a spectrum associated with the transmitted, transflected, or reflected radiation, the spectral response of each of said filters having at least partial overlap with the response of at least one other of said filters, wherein each of said filters is impinged on the radiation transmitted, transflected, or reflected from said sample or standard sequentially and the output from said detector is used to measure the concentration.

24. The system of claim 20, wherein said detector comprises broad bandpass filters.

25. The system of claim 20, wherein the detector includes a black/white detection unit which is responsive to the entire spectrum for which said detector has a spectral response.

26. The system of claim 20, wherein the analyzer includes an artificial analog of a neural network.

27. The system of claim 26, wherein said artificial analog of a neural network is calibrated and trained to process said signals to achieve an analog of color constancy in vision.

28. The system of claim 20, wherein said system is used to non-invasively determine the concentration of the analyte in a human body.

29. The system of claim 20, wherein said analyte is selected from the group consisting of glucose, glucose indicating constituents, hemoglobin, and hemoglobin indicating constituents.

30. The system of claim 20, wherein said analyte is selected from the group consisting of drugs of abuse and drugs of abuse indicating constituents.

31. The system of claim 20, wherein the sample includes a portion of a finger.

32. The system of claim 20, wherein the analyzer obtains the correction vector by utilizing a first vector associated with a first spectral property of the standard in the non-scattering medium;

and a second vector associated with a second spectral property of the standard in a second sample containing the scattering medium.

33. The system of claim 32, wherein the first vector and the second vector both belong to a vector space whose dimension is equal to the number of detection units in the detection array.

34. The system of claim 33, wherein the analyzer calculates a rotation that transforms the second vector into the first vector, said rotation specifying the correction vector.

35. The system of claim 32, wherein the second sample is in vivo.

36. The system of claim 20, wherein the standard in the non-scattering medium is in vitro.

37. The system of claim 20, wherein the sample is in vivo.

38. The system of claim 20, wherein the vector space has a dimension that is equal to the number of detection units.