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Compositions and methods for polynucleotide sequence determination

  • US 6,803,201 B2
  • Filed: 01/24/2002
  • Issued: 10/12/2004
  • Est. Priority Date: 01/24/2002
  • Status: Expired due to Fees
First Claim
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1. A composition for identifying a nucleotide at a predetermined position of a target polynucleotide in a sample, said composition comprising:

  • (a) an oligonucleotide primer comprising a first sequence which hybridizes to said target polynucleotide immediately 3′

    of said nucleotide, and a second sequence which does not hybridize to said target polynucleotide in the presence of a third sequence; and

    (b) an oligonucleotide probe comprising said third sequence which hybridizes to said second sequence of said oligonucleotide primer, said oligonucleotide probe labeled with a first member of a pair of interactive labels.

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