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Arrays of partially nonhybridizing oligonucleotides and preparation thereof using focused acoustic energy

  • US 6,806,051 B2
  • Filed: 09/24/2001
  • Issued: 10/19/2004
  • Est. Priority Date: 09/25/2000
  • Status: Expired due to Term
First Claim
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1. An oligonucleotide array comprised of each attached to a substrate surface through the 3′

  • -hydroxyl group or the 5′

    -hydroxyl group of the oligonucleotide, wherein at least one of the oligonucleotides is a multifunctional oligonucleotide probe comprised of at least two discrete hybridizing segments with a nonhybridizing spacer segment present between any two hybridizing segments, wherein (a) at least two of the hybridizing segments within the same multifunctional oligonucleotide probe are identical, or (b) at least two of the hybridizing segments within the same multifunctional oligonucleotide probe are different and capable of hybridizing to different nucleic acid sequences, such that each hybridizing segment within the same multifunctional probe is capable of use as an individual probe, and further wherein the nonhybridizing spacer segments are selected from nonnucleotidic spacer segments and nucleotidic spacer segments having an equilibrium constant of less than or equal to 1% of the binding constant of the hybridizing segments to complementary oligonucleotide segments in an oligonucleotide analyte.

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