Causative agent of the mystery swine disease, vaccine compositions and diagnostic kits

• US 6,806,086 B2
• Filed: 08/21/2002
• Issued: 10/19/2004
• Est. Priority Date: 06/06/1991
• Status: Expired due to Fees

• Current Assignee: Stichting Dienst Landbouwkundig Onderzoek
• Original Assignee: Stichting Centraal Diergeneeskundig Instituut
• Inventors: Terpstra, Catharinus, Wensvoort, Gert, Meulenberg, Johanna Jacoba Maria, Moormann, Robertus Jacobus Maria, Pol, Joannes Maria Anthonis
• Primary Examiner(s): SALIMI, ALI REZA
• Application Number:
  US10/226,065
• Publication Number:
  US 20030118608A1
• Time in Patent Office: 790 Days
• Field of Search: 435/6, 435/7.9, 435/5, 435/975, 435/7.94, 424/159.1, 424/130.1
• US Class Current: 435/5
• CPC Class Codes: A61K 2039/525 Virus
• CPC Class Codes: A61K 2039/5252 inactivated (killed)
• CPC Class Codes: A61K 2039/5254 avirulent or attenuated
• CPC Class Codes: A61K 2039/53 DNA (RNA) vaccination
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 31/14 for RNA viruses
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C07K 16/10 from RNA viruses
• CPC Class Codes: C12N 2770/10021 Viruses as such, e.g. new isolates, mutants or their genomic sequences
• CPC Class Codes: C12N 2770/10022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2770/10034 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 1/6888 for detection or identification of organisms
• CPC Class Codes: Y10S 424/815 Viral vaccine for porcine species, e.g. swine
• CPC Class Codes: Y10S 435/975 Kit

Abstract

Composition of matter comprising the causative agent of Mystery Swine Disease, Lelystad Agent, in a live, attenuated, dead, or recombinant form, or a part or component of it. Vaccine compositions and diagnostic kits based thereon. Recombinant nucleic acid comprising a Lelystad Agent-specific nucleotide sequence. Peptides comprising a Lelystad Agent-specific amino acid sequence. Lelystad Agent-specific antibodies.

Claims

1. A diagnostic kit for detecting an antibody which specifically recognizes Lelystad Agent in a biological sample, said diagnostic kit comprising

an antigen selected from the group consisting of virus, protein, polypeptide, and peptide, said antigen immunoreactive with serum antibodies of a sow, said serum antibodies obtained by:

a) intranasally inoculating a specific pathogen free sow with two milliliters of a virus essentially corresponding to the virus identified as deposit number I-1102, deposited Jun. 5, 1991 with the Institut Pasteur, Paris, France (at passage level 3, titer 10^4.8 TCID₅₀/milliliter); and

further comprising

b) collecting serum antibodies from the thus inoculated sow after 25 to 33 days, and

means for detecting an antibody.

3. The diagnostic kit of claim 1 wherein the antigen is a live virus.

2. A diagnostic kit for detecting an antibody which specifically recognizes Lelystad Agent in a biological sample said Lelystad Agent essentially corresponding to the isolated Lelystad Agent deposited 5 Jun. 1991 with the Institut Pasteur, Paris, France, deposit number I-1102, said diagnostic kit comprising:

a protein or antigenic polypeptide from Lelystad Agent, and

means for detecting an antibody reacting with said protein or antigenic polypeptide.