Method for removing a fluid substance from a collection device

• US 6,806,094 B2
• Filed: 03/29/2001
• Issued: 10/19/2004
• Est. Priority Date: 05/14/1999
• Status: Expired due to Term

• Current Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Original Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Inventors: Light, James P. II, Kacian, Daniel L., Tseo, Gus G., Carter, Nick M., Anderson, Bruce W., Iheme, Mordi I., Johnson, Shirley J.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): CROSS, LATOYA I
• Application Number:
  US09/821,486
• Publication Number:
  US 20010041336A1
• Time in Patent Office: 1,300 Days
• Field of Search: 436/180, 422/99, 422/100, 422/102
• US Class Current: 436/180
• CPC Class Codes: B01L 2200/026 Fluid interfacing between devices or objects, e.g. connectors, inlet details
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2200/141 Preventing contamination, tampering
• CPC Class Codes: B01L 2300/042 Caps; Plugs
• CPC Class Codes: B01L 2300/044 pierceable, e.g. films, membranes
• CPC Class Codes: B01L 2300/046 Function or devices integrated in the closure
• CPC Class Codes: B01L 2300/047 Additional chamber, reservoir
• CPC Class Codes: B01L 2300/048 enabling gas exchange, e.g. vents
• CPC Class Codes: B01L 2300/0609 Holders integrated in container to position an object
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 3/0275 Interchangeable or disposable dispensing tips
• CPC Class Codes: B01L 3/5029 using swabs
• CPC Class Codes: B01L 3/50825 Closing or opening means, corks, bungs closures for containers B65D; means for removing stoppers B67B7/02
• CPC Class Codes: G01N 35/1079 with means for piercing stoppers or septums
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/25125 Digestion or removing interfering materials
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

The present invention relates to a cap which can form an essentially leak-proof seal with a vessel capable of receiving fluid specimens for clinical analysis and diagnosis. To minimize potentially contaminating contact between the fluid specimen and humans or the environment, the present invention features a cap which is penetrable by a plastic pipette tip or other fluid transfer device, and may include a plurality of striations which were discovered to further improve penetrability of the cap. In this way, substances can be dispensed into or withdrawn from the vessel without having to physically separate the cap from the vessel. Also featured are fluid transfer devices and caps having surface ribs and/or grooves which aid in creating passageways for venting displaced air from a penetrated collection device.

Claims

1. A method for removing a fluid substance from a collection device containing a specimen retrieval device, the method comprising the steps of:

a) positioning at least a portion of the specimen retrieval device along an inner surface of a side wall of a vessel having an open end and a closed end by fixedly associating a penetrable cap with the open end of the vessel, wherein the vessel and the cap compose the collection device;

b) penetrating the cap with a fluid transfer device, wherein the position of the specimen retrieval device within the collection device is such that the specimen retrieval device does not substantially interfere with the movement of the fluid transfer device into or out of the vessel;

c) drawing at least a portion of the fluid substance into the fluid transfer device, wherein the fluid substance is contained in the vessel; and

d) removing the fluid transfer device from the collection device.

2. The method of claim 1, wherein the specimen retrieval device is a swab.

3. The method of claim 2, wherein the swab includes a score line to facilitate snapping-off an upper portion of the swab above the score line.

4. The method of claim 3 further comprising the steps of snapping-off and removing the upper portion of the swab.

5. The method of claim 1, wherein the fluid transfer device is a plastic pipette tip.

6. The method of claim 5, wherein the penetrating step requires applying a force of less than about 8 pounds force to a surface of the cap.

7. The method of claim 1, wherein the cap includes a plurality of striations.

8. The method of claim 1, wherein the fluid substance is suspected of containing a target nucleic acid.

9. The method of claim 8 further comprising the step of amplifying a target nucleic acid sequence contained in the target nucleic acid.

10. The method of claim 9, wherein the amplifying step is performed using a polymerase chain reaction procedure.

11. The method of claim 9, wherein the amplifying step is performed using a transcription-mediated amplification procedure.

12. The method of claim 9 further comprising the step of detecting the presence of the target nucleic acid in the fluid substance.

13. The method of claim 8 further comprising the step of detecting the presence of the target nucleic acid in the fluid substance.

14. The method of claim 1 further comprising the step of inhibiting the release of an aerosol or bubbles from the collection device.

15. The method of claim 14, wherein the inhibiting step is carried out using a wick contained within the cap or an overcap.

16. The method of claim 15, wherein the wick is a pile fabric.

17. The method of claim 9 further comprising the step of inhibiting the release of an aerosol or bubbles from the collection device.

18. The method of claim 17, wherein the inhibiting step is carried out using a wick contained within the cap or an overcap.

19. The method of claim 18, wherein the cap or overcap includes a seal for retaining the wick within the cap or overcap.

20. The method of claim 19, wherein the wick is a pile fabric.