Multi-well rotary synthesizer

• US 6,811,755 B2
• Filed: 07/09/2001
• Issued: 11/02/2004
• Est. Priority Date: 06/16/1998
• Status: Expired due to Term

• Current Assignee: Mcluen Design Incorporated
• Original Assignee: Mcluen Design Incorporated
• Inventors: McLuen, Gary R., Hanney, Richard J., Hugens, Daniel W.
• Primary Examiner(s): STONER, KILEY SHAWN
• Application Number:
  US09/901,949
• Publication Number:
  US 20010051114A1
• Time in Patent Office: 1,212 Days
• Field of Search: 422/131, 422/134, 422/188, 422/189, 422/63-65, 422/81, 422/99, 422/101, 422/102, 422/104, 422/129, 422/130, 935/87, 935/88
• US Class Current: 422/131
• CPC Class Codes: B01J 19/00 Chemical, physical or physico-chemical processes in general; Their relevant apparatus
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00286 Reactor vessels with top and bottom openings
• CPC Class Codes: B01J 2219/00308 interchangeably mounted in racks or blocks
• CPC Class Codes: B01J 2219/00326 Movement by rotation
• CPC Class Codes: B01J 2219/00351 Means for dispensing and evacuation of reagents
• CPC Class Codes: B01J 2219/00353 Pumps
• CPC Class Codes: B01J 2219/00423 using filtration, e.g. through porous frits
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00689 using computers
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor
• CPC Class Codes: Y10T 436/113332 with conveyance of sample along a test line in a container or rack
• CPC Class Codes: Y10T 436/114165 with step of insertion or removal from test line
• CPC Class Codes: Y10T 436/114998 with treatment or replacement of aspirator element [e.g., cleaning, etc.]
• CPC Class Codes: Y10T 436/25375 : Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 : including use of a solid sorbent, semipermeable membrane, or liquid extraction
• CPC Class Codes: Y10T 436/25625 : Dilution
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

An apparatus for synthesizing polymer chains includes a controller, a plurality of precision fit vials circularly arranged in multiple banks on a cartridge, a drain corresponding to each bank of vials, a chamber bowl, a plurality of valves for delivering reagents to selective vials, and a waste tube system for purging material from the vials. A purging operation can be selectively performed on one or more of the banks of vials. The multiple banks of valves provide an additional number of reagent choices while operating in a serial mode and faster reagent distribution while operating in a parallel mode. The plurality of vials are stored in the cartridge and are divided among individual banks wherein each bank of vials has a corresponding drain. There is at least one waste tube system for expelling the reagent solution from vials within a particular bank of vials when the waste tube system is coupled to the corresponding drain. The cartridge holding the plurality of vials rotates relative to the stationary banks of valves and the waste tube system. The controller rotates the cartridge and operates the banks of valves and the waste tube system in response to the required sequence of dispensing various reagent solutions and flushing appropriate vials in order to form the desired polymer chain within each vial.

Claims

1. A synthesizer for forming a polymer chain by sequentially adding monomer units found in one of a plurality of reagent solutions, the synthesizer comprising:

a. a first vial and a second vial, each for holding a selective one or more of the pluralityo f reagent solutions during synthesis;

b. means for dispensing configured for dispensing the selective one or more of plurality of reagent solutions into the first and second vials; and

c. means for selectively expelling material from the first and second vials, configured for engaging a drain associated with a selective one of the first vial and the second vial and thereby purging material from a selective one of the first vial and the second vial when the drain is engaged.

2. The synthesizer according to claim 1 further comprising a cartridge configured for holding the first vial and the second vial.

3. The synthesizer according to claim 2 wherein the cartridge holds the first vial and the second vial along a circular perimeter of the cartridge.

4. The synthesizer according to claim 3 further comprising a motor coupled to the cartridge configured for selectively rotating the cartridge relative to the means for sequentially dispensing in a circular motion.

5. The synthesizer according to claim 4 further comprising a drain plate coupled to the cartridge for separating the first vial into a first bank of vials and the second vial into a second bank of vials.

6. The synthesizer according to claim 1 further comprising a chamber bowl coupled to the means for selectively expelling wherein the chamber bowl contains any spilled material.

7. The synthesizer according to claim 6 further comprising a seal coupled to the chamber bowl to prevent spilled material from escaping outside the chamber bowl.

8. The synthesizer according to claim 1 wherein the means for selectively expelling further comprises:

a. means for forming a pressure differential between a first opening and a second opening of the selective one of the first vial and the second vial; and

b. a waste tube to collect material expelled from the selective one of the first vial and the second vial.

9. The synthesizer according to claim 1 wherein the means for dispensing further comprises:

a. a plurality of valves for controlling dispensing of the plurality of reagent solutions; and

b. a plurality of dispense lines wherein each of the plurality of the dispense lines is coupled to a corresponding one of the plurality of valves for delivering one of the plurality of reagent solutions to a selected vial.

10. The synthesizer according to claim 1 wherein each of the first vial and the second vial further comprise a bored interior having a consistent dimension for maintaining a consistent flow through the first and second vials and are configured to hold a frit for retaining a solid material above the frit.

11. A synthesizer for forming a polymer chain by sequentially adding monomer units found in a plurality of reagent solutions, the synthesizer comprising:

a. a first vial for holding a selective one or more of the plurality of reagent solutions during synthesis;

b. a second vial for holding a selective one or more of the plurality of reagent solutions during synthesis;

c. a cartridge to hold the first vial and the second vial;

d. a dispensing system to dispense the selective one or more of the plurality of reagent solutions into the first and second vial; and

e. a purging system to remove material from a selective one of the first vial and the second vial, wherein the purging system is configured to engage a drain associated with the selected one of the first vial and the second vial and purge material from the selected one of the first vial and the second vial when the drain is engaged.

12. The synthesizer according to claim 11 wherein the cartridge holds the first vial and the second vial along a circular perimeter of the cartridge.

13. The synthesizer according to claim 11 further comprising a chamber bowl coupled to the purging system wherein the chamber bowl contains spilled material.

14. The synthesizer according to claim 13 further comprising a seal coupled to the chamber bowl to prevent spilled material from escaping outside the chamber bowl.

15. The synthesizer according to claim 11 wherein the purging system further comprise:

a. means for forming a pressure differential between a first opening and a second opening of the selective one of the first vial and the second vial; and

b. a waste tube to collect material expelled from the selective one of the first vial and the second vial.

16. The synthesizier according to claim 11 wherein the dispensing system further comprises:

a. a plurality of valves for controlling dispensing of the plurality of reagan solutions; and

b. a plurality of dispense lines each coupled to one of the plurality of valves for delivering a corresponding one of the reagent solutions to a selected vial.

17. The synthesizier according to claim 11 wherein each of the first vial and the second vial further comprise a bored interior having a consistent dimension for maintaining a consistent flow through first and second vials and are configured to hold a frit for retaining a solid material above the frit.

18. The synthesizer according to claim 11 further comprising a drain plate coupled to the cartridge for separating the first vial into a first bank of vials and the second vial into a second bank of vials.

19. The synthesizer according to claim 11 further comprising a motor coupled to the cartridge to selectively rotate the cartridge relative to the dispensing system.

20. synthesizer for creating a polymer chain by sequentially adding monomer units found in one of a plurality of reagent solutions, the synthesizer comprising:

a. a plurality of vials each for holding material including a selective one of the plurality of reagent solutions during synthesis;

b. a cartridge for holding the plurality of vials and dividing the plurality of vials into a first bank of vials including at least one of the plurality of vials and a second bank of vials including at least one of the plurality of vials;

c. a first drain associated with the first bank of vials;

d. a second drain associated with the second bank of vials;

e. a dispensing system configured to sequentially dispense selective ones of the plurality of reagent solutions into the plurality of vials; and

f. a purging system configured to purge material from a selective one of the first bank of vials and the second bank of vials, wherein the purging system is configured to engage the selected one of the first drain and the second drain to purge material.

21. The synthesizer according to claim 20 wherein the purging system further comprises waste tube capable of selectively coupling with a selective one of the first drain and the second drain to purge material from the first bank of vials and the second bank of vials.

22. The synthesizer according to claim 20 wherein the dispensing system further comprises:

a. a plurality of valves for controlling the dispensing of the plurality of reagent solutions; and

b. a plurality of dispense lines each coupled to one of the plurality of valves for delivering a corresponding one of the plurality of regent solutions to a selected vial.

23. A synthesizer for forming a polymer chain by sequentially adding monomer units found in one of a plurality of reagent solution, the synthesizer comprising:

a. a cartridge configured to hold a first vial and a second vial, each for holding a selective one or more of the plurality of reagent solutions during synthesis;

b. a dispensing system configured to dispense the selective one or more of the plurality of reagent solutions into the first and second vials; and

c. a purging system configured to selectively expel material from the first and second vials, wherein a selective one or both of the purging system and the cartridge are moved in relation to each other in order to allow the purging system to engage a drain associated with a selective one of the first vial and the second vial and thereby expel material from to selected one of the first vial and the second vial through the purging system when the drain is engaged.

24. The synthesizer according to claim 23 wherein the cartridge holds the first vial and the second vial along a circular perimeter of the cartridge.

25. The synthesizer according to claim 23 further comprising a motor coupled to the cartridge configured to selectively rotate the cartridge relative to the dispensing system in a circular motion.

26. The synthesizer according to claim 23 wherein the dispensing system further comprises:

a. a plurality of valves for controlling dispensing of the plurality of reagent solutions; and

b. a plurality of dispense lines wherein each of the plurality of the dispense lines is coupled to a corresponding one of the plurality of valves for delivering one of the plurality of reagent solutions to a selected vial.

27. The synthesizer according to claim 23 wherein each of the first vial and the second vial further comprise a bored interior having a consistent dimension for maintaining a consistent flow through the first and second vials and are configured to hold a frit for retaining a solid material above the frit.