Compositions and minimally invasive methods for treating incomplete connective tissue repair

• US 6,811,777 B2
• Filed: 04/11/2003
• Issued: 11/02/2004
• Est. Priority Date: 04/13/2002
• Status: Expired due to Term

• Current Assignee: Blue Engine Biologics, LLC
• Original Assignee: Allan Mishra
• Inventors: Mishra, Allan
• Primary Examiner(s): Saucier, Sandra E.
• Application Number:
  US10/412,821
• Publication Number:
  US 20030194397A1
• Time in Patent Office: 571 Days
• Field of Search: 435/2, 424/93.72
• US Class Current: 424/93.72
• CPC Class Codes: A61K 31/137 Arylalkylamines, e.g. amphetamine, epinephrine, salbutamol, ephedrine or methadone
• CPC Class Codes: A61K 35/14 Blood; Artificial blood perfluorocarbons A61K31/02; umbilical cord blood A61K35/51; haemoglobin A61K38/42
• CPC Class Codes: A61K 35/16 Blood plasma; Blood serum umbilical cord blood A61K35/51
• CPC Class Codes: A61K 35/19 Platelets; Megacaryocytes
• CPC Class Codes: A61K 38/14 Peptides containing saccharide radicals; Derivatives thereof , e.g. bleomycin, phleomycin, muramylpeptides or vancomycin
• CPC Class Codes: A61K 9/0019 Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner non-active ingredients are additionally classified in A61K47/00
• CPC Class Codes: A61P 19/04 for non-specific disorders of the connective tissue
• CPC Class Codes: A61P 21/00 Drugs for disorders of the muscular or neuromuscular system
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: A61P 9/10 for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis

Abstract

Methods are described for using compositions containing platelet-rich plasma for the treatment of a variety of tissue lesions. Particularly, delivery of platelet-rich plasma to connective tissue is described. The described method and compositions have been shown to provide both pain relief and improved mobility in treatment of lateral epicondylitis.

Claims

1. A method of treating an injured tissue in an individual comprising:

determining a site of connective tissue injury in the individual; and

introducing a platelet-rich plasma composition into and around the site of connective tissue injury, wherein no exogenous activator is added to the composition prior to its introduction into and around the site of injury.

2. The method of claim 1, further comprising the step of titrating the platelet-rich plasma composition to obtain a pH of about 7.3 to 7.5.

3. The method of claim 2, wherein the titration is performed using a bicarbonate buffer.

4. The method of claim 1, wherein the platelet-rich plasma composition comprises platelets obtained from the individual.

5. The method of claim 1, further comprising the step of mixing into the platelet composition substantially simultaneously with the introduction into and around the site of tissue injury, one or more pH adjusting agents.