Retentate chromatography—profiling with biospecific interaction adsorbents
First Claim
Patent Images
1. A method for detecting polypeptide analytes in a sample, the method comprising the steps of:
- a) exposing the sample to between 10-10,000 different selectivity conditions at different addressable locations, each selectivity condition defined by a combination of a biospecific interaction adsorbent comprising a polypeptide and an eluant, and wherein exposing comprises (I) contacting the sample with a biospecific interaction adsorbent bound to a substrate, and (II) washing unbound polypeptides from the biospecific interaction adsorbent with the eluant to allow retention of polypeptide analytes on the biospecific interaction adsorbent; and
b) detecting retained polypeptide analytes on each biospecific interaction adsorbent by laser desorption mass spectrometry from each substrate at different addressable locations.
1 Assignment
0 Petitions
Accused Products
Abstract
This invention provides methods of retentate chromatography for resolving analytes in a sample. The methods involve adsorbing the analytes to a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery.
-
Citations
34 Claims
-
1. A method for detecting polypeptide analytes in a sample, the method comprising the steps of:
-
a) exposing the sample to between 10-10,000 different selectivity conditions at different addressable locations, each selectivity condition defined by a combination of a biospecific interaction adsorbent comprising a polypeptide and an eluant, and wherein exposing comprises (I) contacting the sample with a biospecific interaction adsorbent bound to a substrate, and (II) washing unbound polypeptides from the biospecific interaction adsorbent with the eluant to allow retention of polypeptide analytes on the biospecific interaction adsorbent; and
b) detecting retained polypeptide analytes on each biospecific interaction adsorbent by laser desorption mass spectrometry from each substrate at different addressable locations. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
-
Specification