Regeneration of biosensors

• US 6,812,031 B1
• Filed: 05/03/2000
• Issued: 11/02/2004
• Est. Priority Date: 07/09/1997
• Status: Expired due to Fees

• Current Assignee: Senzime AB (publ)
• Original Assignee: Senzime Point of Care AB
• Inventors: Carlsson, Thomas
• Primary Examiner(s): Gakh, Yelena G.
• Application Number:
  US09/462,394
• Time in Patent Office: 1,644 Days
• Field of Search: 436/16, 436/52, 436/95, 436/66, 435/3, 435/7.1, 435/14, 204/400
• US Class Current: 436/52
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: Y10T 436/106664 Blood serum or blood plasma standard or control
• CPC Class Codes: Y10T 436/117497 with a continuously flowing sample or carrier stream

Abstract

The invention comprises a method of regenerating a biosensor. It involves passing a background flow of fluid without reactive components through the flow passage. At a selected point in time a sample aliquot is injected into said background flow. At a point in time when a signal from said sensor is obtained the flow rate of the background fluid is increased. The invention also comprises a system for continuous monitoring of analytes in a biological fluid, the system having increased life by virtue of inherent regeneration of sensors employed. It comprises a biosensor (26, 30, 32), a sampling device (4) for providing a sample of said biological fluid, and means (10, 15, 18, 24) for passing a flow of a background fluid through said flow passage at selectable flow rates, means (20, 50, 55) for injecting said sample into said flow of background fluid, and means (50, 55) for increasing the flow rate of said combined flow. Means for achieving a washing action at the signal generating portion are provided.

Claims

1. A method of regenerating a biosensor of the type having a signal generating portion, wherein said biosensor is responsive to a property of or to the presence of a component in a biological fluid, and has a flow passage in which said signal generating portion is located and through which fluid is passed at selectable flow rates, the method comprising:

(a) passing a background flow of fluid through the flow passage without response generating components;

(b) introducing a sample aliquot into said background flow at a selected point in time; and

(c) increasing the flow rate of the background fluid at a point in time when at least a fraction of said sample aliquot has entered said flow passage in said sensor, such that the rate of increase of the flow rate of the background fluid occurs essentially simultaneously with the entry of the sample aliquot in the flow passage.

2. The method of claim 1, comprising detecting the presence of the sample by the sensor and increasing the flow rate at a point in time 0-30 seconds after the presence of sample is detected, preferably 0-20 seconds, more preferably 0-10 seconds, and most preferably immediately after such detection.

3. The method of claim 1, wherein said flow rate is increased by 5-100%, preferably 10-50%, most preferably 15-30%.

4. The method of claim 1, comprising maintaining the increased flow rate until the signal from the sensor has reached a preselected value.

5. The method of claim 4, wherein said preselected value is a signal peak maximum.

6. The method of claim 1, wherein the increased flow rate is maintained for 10-60 seconds, preferably 20-40 seconds.

7. The method of claim 1, wherein said background flow is 0.1-10 ml/min., preferably 1 ml/min.

8. The method of claim 1, wherein said increase in flow rate is initiated when the entire sample has entered said flow passage.

9. The method of claim 1, wherein sample is continuously drawn from a sample source, and when not being analyzed it is disposed as waste.

10. The method of claim 1, wherein the sample is blood, optionally premixed with anticoagulant.

11. The method of claim 10 wherein said anticoagulant is premixed with blood in a ratio of 1:

1.