Microfluidic system (EDI)

• US 6,812,456 B2
• Filed: 05/24/2002
• Issued: 11/02/2004
• Est. Priority Date: 03/19/2001
• Status: Expired due to Term

• Current Assignee: Gyros Patent AB (Mesa Laboratories Inc.)
• Original Assignee: Gyros AB (Mesa Laboratories Inc.)
• Inventors: Hellermark, Cecilia, Andersson, Per, Palm, Anders, Gustafsson, Magnus, Wallenborg, Susanne
• Primary Examiner(s): Wells, Nikita
• Assistant Examiner(s): Kalivoda, Christopher M.
• Application Number:
  US10/148,083
• Publication Number:
  US 20030066957A1
• Time in Patent Office: 893 Days
• Field of Search: 250/281-300
• US Class Current: 250/288
• CPC Class Codes: B01F 25/433 Mixing tubes wherein the shape of the tube influences the mixing, e.g. mixing tubes with varying cross-section or provided with inwardly extending profiles
• CPC Class Codes: B01F 25/4331 Mixers with bended, curved, coiled, wounded mixing tubes or comprising elements for bending the flow
• CPC Class Codes: B01F 25/4338 Mixers with a succession of converging-diverging cross-sections, i.e. undulating cross-section
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01F 35/711 for feeding a mixture of components, i.e. solids in liquid, solids in a gas stream
• CPC Class Codes: B01F 35/7172 using capillary forces
• CPC Class Codes: B01F 35/71725 using centrifugal forces
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/142 Preventing evaporation
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0806 Standardised forms, e.g. compact disc [CD] format
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/16 Surface properties and coatings
• CPC Class Codes: B01L 2400/0406 : capillary forces
• CPC Class Codes: B01L 2400/0409 : centrifugal forces
• CPC Class Codes: B01L 2400/0418 : electro-osmotic flow [EOF]
• CPC Class Codes: B01L 2400/0633 : with moving parts
• CPC Class Codes: B01L 2400/0688 : surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 3/5025 : for parallel transport of multiple samples
• CPC Class Codes: B01L 3/502715 : characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01N 2035/00504 : combined with carousels
• CPC Class Codes: G01N 33/54366 : Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 35/00069 : whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk
• CPC Class Codes: H01J 49/0418 : for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates
• CPC Class Codes: H01J 49/0431 : for liquid samples interfaces to liquid chromatographs G01N30/7233

Abstract

A microfluidic device in form of a disc comprising an MS-port for presentation of an MS-analyte to an EDI-MS apparatus, said MS-port is a part of a microchannel structure (I) comprising an inlet port for a sample, and comprises an EDI-area having a conductive layer (I) and an EDI-surface from which the MS-analyte is to be desorbed/ionised. The device is characterized in that layer (I) has a conductive connection and/or that there is a calibrator area in the proximity of each of said one, two or more MS-ports. In a typical variant the MS-port is in the form of a depression that is in fluid communication with upstream part of microchannel structure (I).

Claims

1. A microfluidic device in the form of a disc comprising an MS-port for presentation of an MS-analyte to an EDI-MS apparatus, said MS-port comprises;

(a) a part of a microchannel structure comprising at least one inlet port, and

(b) an EDI-area comprising a conductive layer and an EDI-surface from which the MS-analyte is desorbed/ionised, wherein said layer comprises a conductive connection providing voltage and charge transport to the EDI-area or a calibrator area is present in the proximity of said MS-port.

2. The microfluidic device of claim 1, wherein said MS-port comprises a depression in one side of said disc, said depression being in fluid communication with upstream parts of said microchannel structure.

3. The microfluidic device of claim 2, wherein said disc comprises two or more microchannel structures, each of which comprises an MS-port with a depression and an EDI-area comprising a conductive layer and an EDI-surface, the depressions being on the same side of the disc, and the conductive layers of the MS-ports being part of a common conductive layer.

4. The microfluidic device of claim 1, wherein said conductive layer in the MS-port is exposed as an EDI-surface.

5. The microfluidic device of claim 3, wherein the conductive layers of each MS-port is exposed as an EDI-surface, and the common conductive layer extends continuously between the MS-ports and comprises the inner walls of the depressions of the MS-ports.

6. The microfluidic device of claim 1, wherein the MS-port of the microchannel structure is open.

7. The microfluidic device of claim 1, wherein the disc comprises:

a) an axis of symmetry perpendicular to the disc, and

b) two or more of said microchannel structures each of which is oriented radially with a liquid flow direction from one of said at least one inlet ports towards the periphery of the disc.

8. The microfluidic device of claim 7, wherein said one inlet port is a sample inlet port.

9. The microfluidic device of claim 7, wherein said MS-ports are at a larger radial distance from the axis of symmetry than said one inlet ports for each of said two or more of said microchannel structure.

10. The microfluidic device of claim 7, wherein said two or more of said microchannel structures each of which is arranged annularly around the axis of symmetry and said MS-ports are at the same radial distance from the axis of symmetry.

11. The microfluidic device of claim 1, wherein said EDI-MS apparatus is a MALDI-MS apparatus.

12. The microfluidic device of claim 1, wherein said EDI-area is a MALDI-area.

13. The microfluidic device of claim 1, wherein said EDI-surface is a MALDI-surface.

14. The microfluidic device of claim 7, wherein each of the MS-ports comprises a depression in one side of the disc, said side being the same for all of the MS-ports.

15. The microfluidic device of claim 7, wherein said conductive layers are part of a common continuous conductive layer.

16. The microfluidic device of claim 7, wherein the conductive layer of each MS-port is exposed as an EDI-surface.

17. The microfluidic device of claim 7, wherein each MS-port comprises a depression with inner walls, and the conductive layers of the MS-port is exposed as EDI-surfaces and are part of a common continuous conductive layer extending continuously between the MS-ports and comprises the inner walls of the depressions of the MS-ports.

19. The microfluidic device of claim 1, wherein said conductive layer of the EDI-area comprises a conducting metal oxide.

20. The microfluidic device of claim 1, wherein said conductive layer of the EDI-area comprises indium tin oxide.

18. A microfluidic device in the form of a disc comprising an MS-port for presentation of an MS-analyte to an EDI-MS apparatus, said MS-port comprises:

(a) a part of a microchannel structure comprising at least one inlet port, and

(b) an EDI-area comprising a conductive layer and an EDI-surface from which the MS-analyte is desorbed/ionised, wherein said layer comprises a conductive connection providing voltage and charge transport to the EDI-area and a calibrator area is present in the proximity of said MS-port.