Identification and expression of a novel kinesin motor protein

• US 6,815,169 B1
• Filed: 11/28/2000
• Issued: 11/09/2004
• Est. Priority Date: 01/23/1998
• Status: Expired due to Fees

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Goldstein, Lawrence S. B., Sakowicz, Roman
• Primary Examiner(s): Smith, Lynette R. F.
• Assistant Examiner(s): HINES, JANA A
• Application Number:
  US09/724,666
• Time in Patent Office: 1,442 Days
• Field of Search: 435/7.1, 435/7.37, 435/7.7, 435/7.71, 435/7.8, 435/7.9, 435/7.92, 435/7.93, 435/7.94, 435/7.95, 435/29, 435/32, 436/501, 436/512, 436/513, 436/546, 436/547, 436/548, 530/371
• US Class Current: 435/7.1
• CPC Class Codes: C07K 14/37 from fungi

Abstract

The invention provides isolated nucleic acid and amino acid sequences of TL-γ, antibodies to TL-γ, methods of screening for TL-γ modulators using biologically active TL-γ, and kits for screening for TL-γ modulators.

Claims

1. A method for identifying an agent as binding to SEQ ID NO:

1, comprising;

(a) contacting a sample that comprises an agent with an amino acid sequence comprising SEQ ID NO;

1, wherein said contacting is under conditions for binding said agent to said amino acid sequence; and

(b) detecting binding of said agent to said SEQ ID NO;

1, thereby identifying said agent as binding to SEQ ID NO;

1.

2. The method of claim 1, wherein said amino acid sequence comprising SEQ ID NO:

1 is immobilized on a support selected from the group consisting of microtiter plate, array, membrane, and bead.

3. The method of claim 1, wherein said amino acid sequence comprising SEQ ID NO:

1 is immobilized on a support comprising glass, plastic, polysaccharide, nylon, and nitrocellulose.

4. The method of claim 1, wherein said agent is a protein.

5. The method of claim 1, wherein said agent is an oligonucleotide.

6. A method for identifying an agent as binding to amino acids 1 to 357 of SEQ ID NO:

1, comprising;

(a) contacting a sample that comprises an agent with an amino acid sequence comprising amino acids 1 to 357 of SEQ ID NO;

1, wherein said contacting is under conditions for binding said agent to said amino acid sequence; and

(b) detecting binding of said agent to said amino acid sequence, thereby identifying said agent as binding to amino acids 1 to 357 of SEQ ID NO;

1.

7. The method of claim 6, wherein said amino acid sequence comprising amino acids 1 to 357 of SEQ ID NO:

1 is immobilized on a support selected from the group consisting of microtiter plate, array, membrane, and bead.

8. The method of claim 6, wherein said amino acid sequence comprising amino acids 1 to 357 of SEQ ID NO:

1 is immobilized on a support comprising glass, plastic, polysaccharide, nylon, and nitrocellulose.

9. The method of claim 6, wherein said agent is a protein.

10. The method of claim 6, wherein said agent is an oligonucleotide.

11. A method for identifying an agent as binding to amino acids 443 to 601 of SEQ ID NO:

1, comprising;

(a) contacting a sample that comprises an agent with an amino acid sequence comprising amino acids 443 to 601 of SEQ ID NO;

1, wherein said contacting is under conditions for binding said agent to said amino acid sequence; and

(b) detecting binding of said agent to said amino acid sequence, thereby identifying said agent as binding to amino acids 443 to 601 of SEQ ID NO;

1.

12. The method of claim 11, wherein said amino acid sequence comprising amino acids 443 to 601 of SEQ ID NO:

1 is immobilized on a support selected from the group consisting of microtiter plate, array, membrane, and bead.

13. The method of claim 11, wherein said amino acid sequence comprising amino acids 443 to 601 of SEQ ID NO:

1 is immobilized on a support comprising glass, plastic, polysaccharide, nylon, and nitrocellulose.

14. The method of claim 11, wherein said agent is a protein.

15. The method of claim 11, wherein said agent is an oligonucleotide.

16. The method for identifying an agent as binding to amino acids 602 to 784 of SEQ ID NO:

1, comprising;

(a) contacting a sample that comprises an agent with an amino acid sequence comprising amino acids 602 to 784 SEQ ID NO;

1, wherein said contacting is under conditions for binding said agent to said amino acid sequence; and

(b) detecting binding of said agent to said amino acid sequence, thereby identifying said agent as binding to amino acids 602 to 784 of SEQ ID NO;

1.

17. The method of claim 16, wherein said agent said sequence comprising amino acids 602 to 784 of SEQ ID NO:

1 is immobilized on a support from the group consisting of microtiter plate, array, membrane, and bead.

18. The method of claim 16, wherein said agent said sequence comprising amino acids 602 to 784 of SEQ ID NO:

1 is immobilized on a support comprising glass, plastic, polysaccharide, nylon, and nitrocellulose.

19. The method of claim 16, wherein said agent is a protein.

20. The method of claim 16, wherein said agent is an oligonucleotide.